Chaperones and the response to protein misfolding stress

该博士项目提案属于工业生物技术（IB） BBSRC优先领域的职权范围。我们的目标是使用实验室和计算方法的结合，以提供蛋白质错误折叠压力的生物学系统的理解。这将包括定义在不同应力条件下控制聚集体形成和去除的规则和原则，然后可以利用这些规则和原则设计策略来调节聚集体的形成。识别和表征防止蛋白质错误折叠压力的伴侣蛋白将有利于对生物技术和生物制药应用的生物系统中蛋白质产量最大化感兴趣的工业研究人员。这是基础生物科学，将产生适用于酵母细胞培养中重组蛋白的最佳表达的工具。

This PhD Project Proposal falls within the remit of the Industrial Biotechnology (IB) BBSRC priority area. We aim to use a combination of lab and computational approaches to provide a systematic understanding of the biology of protein misfolding stress. This will include defining the rules and principles governing the formation and removal of aggregates under different stress conditions, which can then be exploited to design strategies to mediate against aggregate formation. Identifying and characterizing the chaperones that protect against protein misfolding stress will benefit industrial researchers interested in maximising protein production from biological systems for biotechnological and biopharmaceutical applications. This is fundamental bioscience that will generate tools applicable to the optimal expression of recombinant proteins from yeast cell cultures.