植物在生长发育过程中容易受多种内外因素影响而诱发DNA损伤，该过程影响基因组的稳定性，并构成了粮食作物与经济作物产量下滑的潜在威胁。染色体结构维持复合物SMC5/6能够介导同源重组修复。前期已发现辅助共激活因子ADA2b能够与染色质重塑因子SWI3B协同调控SMC5/6复合物并将其精确定位于DNA双链断裂位点上，但ADA2b自身精确定位于DNA双链断裂位点的分子机制未明。本研究通过筛选与ADA2b互作的染色质相关因子，发现ADIP1与ADA2b相互作用，且初步表明ADA2b在DNA损伤位点的定位依赖ADIP1。本项目拟在此基础上利用遗传学、分子生物学、生物化学、细胞生物学等方法，深入研究ADIP1介导ADA2b在DNA损伤位点定位以参与植物DNA损伤应答的分子机制。本研究有利于揭示植物DNA损伤修复的分子机理，为其他物种的DNA损伤响应的探索提供新思路。

Plants are prone to DNA damage caused by a variety of internal or external factors in the process of growth and development, which affects the stability of genome, and constitutes a potential threat to the decline of grain and cash crop production. The SMC5/6 structure maintenance complex of chromosome regulates the homologous recombination during DNA repair. Previously we have found that the transcriptional coactivator ADA2b and SWI3B co-regulate the SMC5/6 complex and locate it on DSBs, however, it remains unclear how ADA2b is regulated and precisely located to the DSBs. In this study, we found that the chromatin associated factor ADIP1 interacts with ADA2b via yeast two hybrid，and preliminarily showed that the location of ADA2b on the DNA damage sites depended on ADIP1. Thus, this project intends to study the molecular mechanism that the medication of ADA2b by ADIP1 in DNA damage response by using genetics, molecular biology, biochemistry, cell biology and other methods. This study is conducive to revealing the molecular mechanism of plant DNA damage repair and provides new ideas for the exploration of DNA damage response of other species.