Core--Chimerism Analysis and Canine Histocompatibility

该核心旨在满足该计划项目补助金的所有四个研究项目的需求。核心人员包括核心主任、一名全职技术员和一名半职研究技术员。I核心有两个组成部分。一项是提供非清髓性预处理和造血细胞移植后犬（项目1和2）和人类患者（项目3和4）的嵌合体分析。将使用基于聚合酶链反应（PCR）的微卫星标记多态性分析进行这些分析。这些研究将用于监测所有四种移植物的供体植入程度。 研究项目。第二个目标是为项目1和2提供犬组织相容性分型，目的是选择犬白细胞抗原（DLA）相同的同窝供体-受体对进行移植。

This core is designed to meet the needs of all four research projects on this Program Project Grant. The core personnel consist of the Core Director, one full-time Technician and one half-time Research Technician. The I core has two components. One will be to provide chimerism analyses in dogs (Projects 1 and 2) and human patients (Projects 3 and 4) after nonmyeloablative conditioning and hematopoietic cell transplantation. These analyses will be performed using polymerase chain reaction (PCR)-based microsatellite marker polymorphism assays. These studies will serve to monitor the degree of donor engraftment in all four research projects. The second goal will be to provide canine histocompatibility typing for Projects 1 and 2 with the aim of selecting dog leukocyte antigen (DLA) identical littermate donor-recipient pairs for transplantation.