Micromachined Si Printhead for Microarray Fabrication

用于微阵列制造的微机械硅打印头

• 批准号: 7117269
• 负责人: ROBERT C HAUSHALTER
• 金额: $ 27.57万
• 依托单位: PARALLEL SYNTHESIS TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7117269
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biotechnology; gene expression; high throughput technology; microarray technology; printing; silicon

DESCRIPTION (provided by applicant): During the Phase I effort, a totally new type of passive microcontact printing tool was developed by Parallel Synthesis Technologies that can print microarrays of a quality, cost and speed previously unobtainable. Silicon micromachining techniques were employed to fabricate a robust, inexpensive and very high density "printing microtiter plate" which prints sub-nL individual samples from each well onto a microarray format. The technology is based on arrays (1536 or 6144 SBS format) of individually compliant, silicon-spring loaded, hollow bore nozzles, with print tips in the range of 25-1 OOum connected to reservoir wells of volume about 0-5 uL, which are filled by automated liquid dispensers. Very large numbers (up to 10,000 spots/tip/loading) of extremely uniform spots (%CV spot size for one pin approximately 2% and 100 pins is approximately 5%) can be rapidly printed at an estimated rate of 1-10 x 10^6 spots/hour/head with less than <0.07% missing spots. In Phase II, two print heads of full microscope slide size (25 x 75 mm) will be fabricated at 1536 to 6144 formats to give a total of 300 or 1200 tips/print head, respectively. A proposed printer design, where one of these arrays is selected from among many in a carousel, should allow microarray printing at an unprecedented rate. After fabrication and testing at Parallel, the print heads will be distributed to three collaborators to determine if this technology will produce DNA and protein microarrays of an improved quality in three different array fabrication settings. The parallel print head will be employed (a) to print a 44,000 spot viral DNA array in an academic research setting with Prof. J. DeRisi (UCSF), (b) to print several hundred spot prototype diagnostic arrays with Dr. T. Tisone (Biodot, Inc.) and (c) to print antibody arrays in microtiter plate formats with Dr. D. Bochkariov (BD - Clontech). If these printing tools provide the anticipated large improvements, then the testing, sale and distribution can employ the existing channels currently used by Parallel for its commercially successful Silicon Microarray(tm) technology based on individual silicon pins which has been very well received by the microarray community. A fundamentally new type of multilevel silicon etch process was discovered during the course of this work and a patent application is in preparation.

描述（由申请人提供）：在第一阶段的努力中，平行合成技术公司开发了一种全新的无源微接触打印工具，可以打印质量，成本和速度以前无法实现的微阵列。硅微加工技术被用来制造一个坚固的、廉价的和非常高密度的“印刷微量滴度板”，从每个孔中打印出低于nl的单个样品到微阵列格式。该技术基于阵列（1536或6144 SBS格式），单个兼容，硅弹簧加载，中空孔喷嘴，打印尖端范围为25-1 oum，连接到容量约为0-5 uL的储层井，由自动液体分配器填充。非常大量（高达10,000个点/针尖/负载）非常均匀的斑点（一个引脚的%CV斑点尺寸约为2%，100个引脚约为5%）可以以估计的1-10 x 10^6点/小时/头的速度快速打印，缺失点小于<0.07%。在第二阶段，两个全显微镜载玻片尺寸的打印头（25 x 75 mm）将以1536至6144格式制造，分别提供总共300或1200个打印头。一个提议的打印机设计，其中一个这些阵列是在一个传送带中选择的，应该允许微阵列打印以前所未有的速度。在Parallel公司完成制作和测试后，打印头将分配给三位合作者，以确定该技术是否能在三种不同的阵列制作设置下生产质量更高的DNA和蛋白质微阵列。平行打印头将用于(a)与J. DeRisi教授（UCSF）在学术研究环境中打印44,000个点病毒DNA阵列，(b)与T. Tisone博士（Biodot, Inc.）打印数百个点原型诊断阵列，(c)与D. Bochkariov博士（BD - Clontech）在微滴板格式中打印抗体阵列。如果这些印刷工具提供了预期的巨大改进，那么测试、销售和分销可以采用Parallel公司目前使用的现有渠道，该公司基于单个硅引脚的硅微阵列（tm）技术在商业上取得了成功，该技术已经得到了微阵列社区的好评。在这项工作的过程中，发现了一种全新的多层硅蚀刻工艺，并正在准备专利申请。