Investigating the transcriptional and functional stress responses in different neuron subtypes after knockdown of mitochondrial complex I subunit ND-7

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We hypothesise that mitochondrial complex I inhibition (via knock down of subunit ND-75) will lead to distinct transcriptional and functional stress responses in different neuronal subtypes. To test this hypothesis in the next upcoming months I plan to:1. Analyze the behavioural effects of ND-75 KD in a single neuronal subtype, dopaminergic and serotonergic neurons.Climbing and seizure assaysCAFÉ feeding assayANY-Maze open field assayHot plate assayAnalysis of ATF4 expression- adult brain dissections, staining and confocal microscopy 2. Analyze the transcriptional response caused by ND-75 KD in single neurons. -We will perform single nuclear RNA-seq (snRNA-seq) from flies with pan-neuronal ND-75 KD and ND-75 KD together with ATF4 KD.3. Test whether targeting mitochondrial stress signalling modifies dopaminergic and serotonergic neuron function.