Proteomics Core

蛋白质组学核心

• 批准号: 7185716
• 负责人: Joseph A Loo
• 金额: $ 17.53万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7185716
• 关键词: air pollution; airborne allergen; asthma; bioinformatics; biomedical facility; biotechnology; engine exhaust; inflammation; mass spectrometry; monocyte; oxidative stress; pollution related respiratory disorder; posttranslational modifications; proteomics; respiratory hypersensitivity; two dimensional gel electrophoresis

The Proteomics Core supports the research goals of the Asthma Allergic Disease Research Center (AADRC) by providing state-of-the-art mass spectrometry and expertise for the characterization of proteins and their post-translational modifications such as phosphorylation, and profiling changes in protein expression. The AADRC is involved in studying the adjuvant effects of diesel exhaust particles (DEP) and concentrated air particles in allergic airway inflammation and asthma. The Core, centrally located for UCLA researchers in the Molecular Sciences Building, houses a wide range of equipment for two dimensional gel electrophoresis protein separation, mass spectrometry-based protein characterization, and software and bioinformatics to facilitate protein identification. The Proteomics Core, under the leadership of Dr Joseph Loo, will facilitate the proteome analysis on: (i) bronchoalveolar lavage fluid with a view to demonstrating PM-induced oxidative stress responses and the role of phase II enzymes in allergic inflammation, (ii) characterize the proteome of human nasal lavage fluid to measure PM-induced oxidative stress responses in human subjects with high and low responses to DEP and to measure responses from subjects showing potent sulforaphane-induced protection from DEP and cat allergen exposure, and (iii) perform proteome analysis on human peripheral blood monocytes to confirm the genetic response differences between the high and low responder subsets. Proteomic approaches will be applied to develop a multi-pronged strategy to assemble the most complete picture of the cellular, molecular, and biochemical mechanisms of particular pollution enhancement of allergic inflammation. Relevance: The Proteomics Core houses the necessary technology to monitor the changes in protein expression measured in body fluids, tissues, and cells that result from exposure to particulate pollution. The goal of the Core is to find the molecular and biochemical link between the adverse health effects of particulate pollution and allergic inflammation.

蛋白质组学核心支持哮喘过敏性疾病研究中心的研究目标 （AADRC）通过提供最先进的质谱和蛋白质表征的专业知识 以及它们的翻译后修饰，如磷酸化，以及蛋白质中的谱变化， 表情AADRC参与研究柴油机排气颗粒（DEP）的辅助作用， 过敏性气道炎症和哮喘中的浓缩空气颗粒。核心，位于加州大学洛杉矶分校的中心 研究人员在分子科学大楼，房屋的二维凝胶广泛的设备 电泳蛋白质分离、基于质谱的蛋白质表征和软件， 生物信息学，以促进蛋白质鉴定。蛋白质组学核心，在约瑟夫博士的领导下 Loo，将有助于对以下内容进行蛋白质组学分析：（i）支气管肺泡灌洗液，以证明 PM诱导的氧化应激反应和II相酶在过敏性炎症中的作用，（ii） 表征人鼻灌洗液的蛋白质组以测量PM诱导的氧化应激反应， 具有对DEP的高和低响应的人类受试者，并测量来自显示 有效的萝卜硫素诱导的保护，从DEP和猫过敏原暴露，和（iii）进行蛋白质组 分析人外周血单核细胞，以确认 高和低应答者子集。蛋白质组学方法将被应用于开发多管齐下的策略 组装的细胞，分子和生化机制的最完整的图片，特别是 污染增强过敏性炎症。 相关性：蛋白质组学核心包含必要的技术来监测蛋白质的变化， 在暴露于颗粒污染的体液、组织和细胞中测量的表达。的 核心的目标是找到分子和生物化学之间的联系， 微粒污染和过敏性炎症。