MALDI TOF/TOF Mass Spectrometer for Imaging Thin Tissue Sections

描述(由申请人提供)： 我们正在申请资助最先进的基质辅助激光解吸电离飞行时间/飞行时间(MALDI-TOF/TOF)质谱仪，用于薄组织切片的质谱学成像。蛋白质组学技术的一个令人振奋的新应用是利用MALDI-TOF/TOF质谱仪(MS)对组织进行分子成像。直接组织图谱和成像质谱仪提供对组织样本中复杂蛋白质模式的详细评估，允许可视化蛋白质/多肽和其他分析物在组织切片中的空间分布，并深入了解特定的细胞和组织生物学和病理学。这项新开发的技术需要高灵敏度的质谱仪，我们将利用质谱仪的能力，既可以在线性模式下工作，也可以在反射模式下工作，以获得高分辨率和高质量精度。该仪器的MS/MS测序能力将允许直接从组织中识别临床相关的多肽，而不需要进一步的分离或分离。MALDI的组织成像技术允许以高通量方式自动处理临床样本，并扩大了临床蛋白质组学核心、组织采购核心实验室和加州大学洛杉矶分校分子仪器中心的研究能力。MALDI-TOF/TOF成像系统将是加州大学洛杉矶分校NIH资助的10多个不同项目的重要组成部分。这项受资助的研究将影响一些以生物医学为导向的研究，包括癌症(前列腺癌、脑癌、皮肤癌和肺癌)、免疫/炎症性疾病(炎症性肠道疾病)、代谢性疾病(糖尿病和代谢综合征)和心血管疾病(动脉粥样硬化)。 公共卫生相关性：新的仪器将有利于广泛的面向临床的研究，特别是在转化医学领域。我们将在一系列重要的人类疾病中寻找新的生物标记物，包括前列腺癌和肺癌、胶质母细胞瘤、黑色素瘤和炎症性肠病。我们的研究将寻求识别新的候选生物标记物以及更好地了解疾病进展的病因学和病理学。

DESCRIPTION (provided by applicant): We are requesting funding for a state-of-the-art matrix-assisted laser desorption ionization time-of-flight/time- of-flight (MALDI-TOF/TOF) mass spectrometer to be utilized for imaging by mass spectrometry (ImMS) of thin tissue sections. An exciting new application of proteomics technology is molecular imaging of tissues by MALDI-TOF/TOF mass spectrometry (MS). Direct tissue profiling and imaging mass spectrometry provide a detailed assessment of the complex protein pattern within a tissue sample, allows visualization of the spatial distribution of proteins/peptides and other analytes in tissue sections, and insight into specific cell and tissue biology and pathology. This newly developed technique requires high sensitivity mass spectrometry and we will be taking advantage of the ability of the mass spectrometer to operate in both linear mode for sensitivity and reflectron mode for high resolution and mass accuracy. The MS/MS sequencing capabilities of the instrument will allow the identification of clinically relevant peptides directly from the tissues without further fractionation or isolation. Tissue imaging by MALDI allows for automated processing of clinical specimens in a high-throughput fashion and expands the research capacity of the Clinical Proteomics Core, the Tissue Procurement Core Laboratory, and the Molecular Instrumentation Center at UCLA. The MALDI-TOF/TOF imaging system will be an important part of over 10 different NIH-funded projects at UCLA. The funded research will impact a number of biomedically-oriented studies including cancer (prostate, brain, skin, and lung cancers), immune/inflammatory disorders (inflammatory bowel disease), metabolic disease (diabetes and metabolic syndrome), and cardiovascular disease (atherosclerosis). PUBLIC HEALTH RELEVANCE: The new instrumentation will benefit a wide range of clinically-oriented studies, particularly in the field of translational medicine. We will be seeking new biomarkers in an array of important human conditions including prostate and lung cancer, glioblastoma, melanoma, and inflammatory bowel disease. Our studies will seek both to identify new biomarker candidates as well as to better understand the etiology and pathology of disease progression.