Development and evaluation of New DNP Probes for Biological Processes by MAS-DNP NMR.

通过 MAS-DNP NMR 开发和评估用于生物过程的新型 DNP 探针。

• 批准号: 2785684
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2785684
• 关键词: Development; evaluation; New; DNP; Probes

Magic-Angle-Spinning Dynamic-Nuclear-Polarization (MAS-DNP) solid-state NMR is beginning to be used to study complex biological structures and biomaterials. Kockenberger/Thomas propose to undertake pioneering studies that are intractable using other methods:A. Using the polarization agent AMUPol, the structure of the crystalline and amorphous regions of 4RepCT spider silk will be studied to identify key aa interactions and correlate these with mechanical properties.B. 4RepCT incorporating L-azidohomoalanine will be conjugated with Gd(III)DOTA/nitroxide as polarization sources to study fibre self-assembly.C. A new targeted polarization strategy will be developed, based on the cell-wall binding domain of lysostaphin-Gd(III)DOTA to probe S. aureus cell envelopes.

魔角旋转动态核极化（MAS-DNP）固态核磁共振技术开始被用于研究复杂的生物结构和生物材料。Kockenberger/托马斯建议进行开创性的研究，这是难以使用其他方法：A。使用极化剂AMUPol，将研究4 RepCT蜘蛛丝的结晶和非晶区域的结构，以确定关键的aa相互作用，并将其与机械性能相关联。4 RepCT掺入L-叠氮基高丙氨酸将与Gd（III）DOTA/氮氧化物缀合作为偏振源以研究纤维自组装。基于溶葡萄球菌酶-Gd（III）DOTA的细胞壁结合结构域，将开发一种新的靶向极化策略以探测S。金黄色细胞被膜。