The Physiological Role of Carbonic Anhydrases in the Ret

碳酸酐酶（CA）催化CO2的可逆水合作用，并间接参与水、碳酸氢盐和质子的运输。用RT-PCR和定量RT-PCR方法在人胚胎RPE细胞中鉴定出12种同工酶。我们已经确定了CA II，IV，IX和XIV在hfRPE细胞的表达和亚细胞分布的免疫细胞化学和Western印迹。免疫细胞化学研究表明CA IV和CA IX在RPE的顶端表面表达。CA II是一种细胞内蛋白，CA XIV主要在侧膜上观察到，也存在于顶膜和基底膜上。用电容探针技术研究了体外培养的人胎儿和成年牛视网膜色素上皮细胞单层的跨上皮液体转运。多佐胺是CA II和IX的CA抑制剂，当应用于细胞的顶端表面时，可引起人胎牛和成年牛RPE的液体吸收可逆性降低。该数据表明CA对于调节穿过RPE的流体运输是重要的。进一步的研究将确定这些CA中的哪一个有助于调节pH值和通过RPE的液体吸收。

Carbonic anhydrases (CAs) catalyze the reversible hydration of CO2 and are indirectly involved in the transport of water, bicarbonate, and protons. Twelve of the fourteen isozymes have been identified by RT-PCR and quantitative RT-PCR in human fetal RPE cells. We have determined the expression and subcellular distribution of CA II, IV, IX, and XIV in hfRPE cells by immunocytochemistry and Western blot. Immunocytochemical studies indicate that CA IV and CA IX are expressed at the apical surface of the RPE. CA II is an intracellular protein and CA XIV is mainly observed on the lateral membrane and is also present at the apical and basal membranes. The capacitance probe technique was used to study transepithelial fluid transport across monolayers of cultured human fetal and adult bovine RPE. Dorzolamide, a CA inhibitor of CA II and IX, caused a reversible decrease in fluid absorption across human fetal and adult bovine RPE when applied to the apical surface of the cells. This data indicates that CAs are important for regulating fluid transport across the RPE. Further studies will determine which of these CAs help regulate pH and absorption of fluid across the RPE.