Forensic Detection of Salmonella Resistance and Subtype

描述(由申请人提供)：非伤寒沙门氏菌肠炎亚种肠杆菌(S.enterica)是全球食源性胃肠炎暴发的主要原因，已被用于生物恐怖主义。此外，全球对抗生素的耐药性有所增加，包括整合子相关的多重耐药。由于肠链球菌感染对军人和平民人口构成持续的生物恐怖威胁，这些发现令人关切。因此，快速检测疫情和耐药性，以及对用于生物恐怖主义的菌株进行法医分析，对公共安全至关重要。该项目建议开发快速的分子方法来监测、检测和描述肠杆菌的暴发。这一建议的核心是识别整合子介导的耐药决定因素，并开发一种虚拟表型方法来检测多重耐药肠球菌。该项目的具体目标是开发和验证1)标准化、高通量的方法，用于快速检测和鉴定整合子介导的肠杆菌多药耐药；2)分子方法，用于肠杆菌血清型的快速暴发检测。

DESCRIPTION (provided by applicant): Non-typhoidal Salmonella enterica subspecies enterica (S. enterica) is responsible for a substantial proportion of global food-borne gastroenteritis outbreaks and has already been used for bioterrorism. In addition, there has been a worldwide increase in antibiotic-resistant S. enterica, including integron-associated multi-drug resistance. These findings are of concern due to the ongoing bioterrorist threat that S. enterica infection poses to military and civilian populations. Therefore, rapid detection of outbreaks and drug resistance, as well as forensic analysis of strains used for bioterrorism, are critical to public safety. This project proposes to develop rapid molecular methods for the surveillance, detection and characterization of S. enterica outbreaks. Central to this proposal is the identification of integron-mediated drug resistance determinants and the development of a virtual phenotype approach for the detection of multi-drug resistant S. enterica. The specific aims of the project are to develop and validate 1) standardized, high-throughput methods for rapid detection and characterization of integron-mediated MDR in S. enterica and 2) molecular methods for rapid outbreak detection among S. enterica serovars.