Engineered enzymes, metabolic pathways and bioproduction systems for high-value chemicals manufacturing.

用于高价值化学品制造的工程酶、代谢途径和生物生产系统。

• 批准号: 2827599
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2827599
• 关键词: Engineered; enzymes; metabolic; pathways; bioproduction

Our aim is to develop renewable chemicals manufacturing that be translated into commercial reality. We are targeting high-value aroma compounds as initial beachhead products. We identified a small number of potential targets with an unmet market need. Candidate enzymes have been identified and will be optimised by targeted mutagenesis through in vivo screening. Subsequently, complete metabolic pathways will be assembled and verified. Thereafter, the aim is to optimise the bioproduction strains utilising Design of Experiment targeting selected factors (protein choice, protein expression, substrate, environment); best solutions are thereafter combined and scaled up in 1L bioreactors with the aim to capture and purify first samples.

我们的目标是发展可再生化学品制造，并将其转化为商业现实。我们的目标是高价值的芳香化合物作为最初的滩头产品。我们确定了少数未满足市场需求的潜在目标。候选酶已经确定，并将通过体内筛选的靶向诱变进行优化。随后，完整的代谢途径将被组装和验证。之后，目标是利用针对选定因素（蛋白质选择，蛋白质表达，底物，环境）的实验设计来优化生物生产菌株；然后将最佳溶液组合并在1L生物反应器中放大，目的是捕获和纯化第一批样品。