Microcarriers designed for protein & sera free media

专为蛋白质设计的微载体

• 批准号: 7039231
• 负责人: William J Hillegas
• 金额: $ 35.9万
• 依托单位: SOLOHILL ENGINEERING, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7039231
• 关键词: animal tissue; bioengineering /biomedical engineering; biological transport; biomaterial development /preparation; biotechnology; cell line; collagen; fibroblasts; human tissue; packaging material; polystyrenes; protein transport; surface coating; synthetic peptide

DESCRIPTION (provided by applicant): The overall goal of this SBIR Phase II application is to develop a family of microcarrier products that have a synthetic or recombinant collagen peptide bound to the bead surface. These microcarrier products would replace current microcarriers that have porcine collagen (i.e., gelatin) as part of the attachment surface. The synthetic collagen peptide-coated microcarriers would be an ideal replacement for existing products in animal product-free manufacturing protocols. Currently, three collagen-coated microcarriers, (a collagen-coated dextran bead [Cytodex 3] manufactured offshore and SoloHill's two gelatin-coated microcarriers) are the most extensively used microcarrier substrates in the industrial scale production of anchorage-dependent cells. As manufacturers of viral vaccines and other biologicals move toward complete animal product-free manufacturing conditions, a substrate containing collagen or gelatin will not be acceptable. Microcarriers with a synthetic collagen peptide - coated surface would provide an ideal replacement. The Phase II studies described here follow directly from the successes achieved in Phase I. The Phase II work plan is divided into three overall specific aims. Specific Aim I. To develop a microcarrier that has a synthetic collagen peptide on its surface. This microcarrier would be an animal product-free equivalent of (and in some applications a replacement for) the SoloHill gelatin-coated microcarriers. Specific Aim II. To develop a dextran microcarrier that has a synthetic collagen peptide on its surface. This microcarrier would be an animal product-free equivalent of (and a replacement for) the collagen-linked dextran microcarriers now manufactured and sold by a foreign competitor. Specific Aim IIl. To investigate the utility of additional microcarrier synthetic surface coatings as replacements of current products with porcine gelatin on the surface. Examples are modifications to the current synthetic collagen peptide and recombinant human collagen and gelatins.

描述（由申请人提供）：该SBIR II期申请的总体目标是开发一系列微载体产品，这些产品具有与微珠表面结合的合成或重组胶原蛋白肽。这些微载体产品将取代目前含有猪胶原蛋白的微载体（即，明胶）作为附着表面的一部分。合成的胶原蛋白肽包被的微载体将是现有产品的理想替代品，在无动物产品的生产方案。目前，三种胶原蛋白包被的微载体（离岸制造的胶原蛋白包被的葡聚糖珠[Cytodex 3]和SoloHill的两种明胶包被的微载体）是在锚定依赖性细胞的工业规模生产中最广泛使用的微载体基底。随着病毒疫苗和其他生物制品的生产商逐渐转向完全不含动物产品的生产条件，含有胶原蛋白或明胶的基质将不可接受。具有合成胶原蛋白肽涂层表面的微载体将提供理想的替代品。本文所述的II期研究直接源自I期研究取得的成功。第二阶段工作计划分为三个总体具体目标。 具体目标一。开发一种表面含有合成胶原肽的微载体。该微载体将是SoloHill明胶涂覆的微载体的无动物产品等同物（并且在某些应用中是SoloHill明胶涂覆的微载体的替代物）。 具体目标二。研制一种表面含有合成胶原肽的葡聚糖微载体。这种微载体将是一种不含动物产品的替代品，相当于目前由外国竞争对手生产和销售的胶原蛋白连接葡聚糖微载体。 具体目标III.研究额外的微载体合成表面涂层作为表面上具有猪明胶的当前产品的替代品的效用。实例是对当前合成胶原蛋白肽和重组人胶原蛋白和明胶的修饰。