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Microcarriers designed for protein & sera free media

专为蛋白质设计的微载体

基本信息

批准号：
6739866
负责人：
William J Hillegas
金额：
$ 9.81万
依托单位：
SOLOHILL ENGINEERING, INC.
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-01-01 至 2004-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6739866
关键词：
alternatives to animals in research bioreactors cell line cell proliferation collagen cost effectiveness dextrans gelatin microcapsule polystyrenes protein sequence synthetic peptide technology /technique development

项目摘要

DESCRIPTION (provided by applicant): The overall goal of this study is to develop a microcarrier coated with a synthetic, 15 amino acid peptide sequence found in type I collagen. This microcarrier would replace current microcarriers utilizing native collagen or gelatin in protocols requiring animal product-free conditions. Currently three gelatin-coated microcarriers, a gelatin-linked dextran (Cytodex III, made by the United Kingdoms's Amersham-Pharmacia Biotechnology) and Solohill Engineering's two gelatin-coated polystyrene microcarriers, are the most extensively used microcarrier substrates in the industrial scale production of anchorage-dependent cells today. As manufacturers of viral vaccines and other biologicals move toward completely animal product-free manufacturing conditions, a substrate containing collagen or gelatin will not be acceptable. The synthetic-collagen-peptide coated microcarriers would provide an ideal replacement. In the Phase I portion of this SBIR grant application, we propose the following experimental program to demonstrate the feasibility of producing such a product. Specific Aim I. To attach a synthetic-collagen-peptide to the surface of polystyrene microcarrier core beads and to compare the synthetic peptide - coated microcarriers with currently available collagen-coated polystyrene microcarriers for ability to support attachment and growth of anchorage-dependent animal cells under serum-free conditions. Specific Aim II. To attach a synthetic-collagen-peptide to the surface of charged-surface polystyrene microcarrier core beads (Hillex microcarriers) and to compare the synthetic peptide-coated microcarriers with currently available charged-surface polystyrene microcarriers for ability to support attachment and growth of anchorage-dependent animals cells under serum-free conditions. Specific Aim III. To attach a synthetic-collagen-peptide to the surface of dextran microcarrier core beads and to compare the synthetic peptide-coated microcarriers with currently-available collagen-coated dextran microcarriers for ability to support attachment and growth of anchorage-dependent animals cells under serum-free conditions.

描述(申请人提供)：这项研究的总体目标是开发一种包被合成的、在I型胶原中发现的15个氨基酸的肽序列的微载体。在要求无动物产品的条件下，这种微载体将取代目前使用天然胶原或明胶的微载体。目前，三种明胶涂层微载体，一种明胶连接的葡聚糖(Cytodex III，由英国的amersham-Pharmacia生物技术公司制造)和SoloHill Engineering的两种明胶涂层聚苯乙烯微载体，是目前工业规模生产锚定依赖细胞中使用最广泛的微载体底物。随着病毒疫苗和其他生物制品的制造商走向完全不含动物产品的生产条件，含有胶原或明胶的底物将是不可接受的。人工合成胶原肽包裹的微载体将是一种理想的替代材料。在这项SBIR赠款申请的第一阶段，我们提出了以下实验计划，以证明生产这种产品的可行性。具体目的1.将合成的胶原肽附着在聚苯乙烯微载体核心小球的表面，并比较合成的多肽涂层微载体与目前可用的胶原涂层聚苯乙烯微载体在无血清条件下支持锚定依赖的动物细胞附着和生长的能力。具体目的II.将合成的胶原肽附着到带电表面聚苯乙烯微载体核心小球(Hillex微载体)的表面，并比较合成的多肽涂层微载体与现有的带电表面聚苯乙烯微载体在无血清条件下支持锚定依赖的动物细胞附着和生长的能力。具体目的III.将合成的胶原肽附着在葡聚糖微载体核心小球的表面，并比较合成的多肽包裹的微载体和目前可用的胶原包裹的葡聚糖微载体在无血清条件下支持锚定依赖的动物细胞附着和生长的能力。