A Singlet Molecular Oxygen Imaging Sensor for Photodynamic Therapy

用于光动力治疗的单态分子氧成像传感器

• 批准号: 7219546
• 负责人: SEONKYUNG LEE
• 金额: $ 14.91万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219546
• 关键词: neoplasm /cancer; oxygen; singlet oxygen; university

DESCRIPTION (provided by applicant): Project Summary/Abstract: Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photosensitizers such as hematoporphyrin derivative (HPD) and other compounds to be selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in the metastable singlet delta state O2(1?) are believed to be the species that destroys cancerous cells during PDT. In this Phase I proposal, Physical Sciences Inc. (PSI) proposes to develop an optically-based monitor that will image the spatial extent and location of both the photosensitizer and singlet oxygen emission. The system will build upon our recent results that have demonstrated the ability to observe singlet oxygen optical emission in-vivo produced during PDT. The system will use a fiber coupled, pulsed diode laser and a state-of-the-art, just commercially introduced 2-D imaging camera to develop and demonstrate spatial imaging of photosensitizer concentration and singlet oxygen production during PDT. The system will be developed and tested on in-vitro samples at PSI then transported to Dartmouth College for in-vivo testing in collaboration with our Phase I partners. The goal of these tests is to demonstrate that the Phase I imaging device can map the spatial location of the singlet oxygen production within the tumors and correlate it with the spatial distribution of the photosensitizer. In Phase II, we will design, build, and test a portable, ruggedized instrument through extensive tests both at Dartmouth College and the Massachusetts General Hospital. A fully developed instrument will be a valuable tool for several aspects of PDT research including: elucidating kinetic and physiological phenomena, assessing new photosensitizers targeted to specific tissue areas, correlating photosensitizer concentrations with singlet oxygen production, and discerning mechanisms of tumor necrosis. Project Narrative: Relevance to public health: This project will demonstrate a novel diagnostic for improving the treatment of cancer by light activated cancer killing drugs. A successful program will produce high resolution images of the location of an excited form of oxygen (produced by the light activated drugs within tumors) that is responsible for killing the tumor. It will be a valuable research tool to develop new, more effective treatments.

描述（由申请人提供）：项目摘要/摘要：光动力疗法（PDT）是一种很有前途的癌症治疗方法。PDT利用光敏剂如血卟啉衍生物（HPD）和其他化合物的亲和力选择性地保留在恶性肿瘤中。当用光敏剂预处理的肿瘤用可见光照射时，发生光化学反应并破坏肿瘤细胞。氧分子在亚稳态单重δ态O2（1？）被认为是在PDT过程中破坏癌细胞的物质。在第一阶段的提案中，物理科学公司。(PSI)提出开发一种基于光学的监测器，该监测器将对光敏剂和单线态氧发射的空间范围和位置进行成像。该系统将建立在我们最近的结果，已经证明了在PDT过程中产生的单线态氧光发射在体内观察的能力。该系统将使用光纤耦合脉冲二极管激光器和刚刚商业化推出的最先进的2-D成像相机来开发和演示PDT期间光敏剂浓度和单线态氧产生的空间成像。该系统将在PSI进行体外样本开发和测试，然后运送到达特茅斯学院与我们的第一阶段合作伙伴进行体内测试。这些测试的目的是证明I期成像设备可以映射肿瘤内单线态氧产生的空间位置，并将其与光敏剂的空间分布相关联。在第二阶段，我们将设计、制造和测试一种便携式、坚固耐用的仪器，并在达特茅斯学院和马萨诸塞州总医院进行广泛的测试。一个完全开发的仪器将是一个有价值的工具，PDT研究的几个方面，包括：阐明动力学和生理现象，评估新的光敏剂针对特定的组织区域，相关的光敏剂浓度与单线态氧的生产，并辨别肿瘤坏死的机制。项目叙述：与公共卫生的相关性：该项目将展示一种新的诊断方法，用于通过光激活抗癌药物改善癌症的治疗。一个成功的程序将产生高分辨率图像的位置的激发形式的氧气（产生的光激活药物在肿瘤内），负责杀死肿瘤。这将是一个有价值的研究工具，以开发新的，更有效的治疗。