Rapid, Accurate, and Simple-to-Use Test Strip for GHB

描述（由申请人提供）：γ-羟基丁酸盐（GHB）作为一种娱乐性药物的滥用正变得越来越普遍，因为它在摄入适度水平时会产生欣快感。在更高的水平上，无意识和昏迷可能导致使其成为“约会强奸”犯罪的首选药物。因此，美国缉毒署已宣布其为附表一受控物质。该提案的目标是创建一个快速，准确，易于使用的测试条能够检测GHB在体液中。目前还不存在这样的测试条，因此当不知道中毒剂的性质时，急诊室人员和急救服务提供者难以定制他们对中毒患者的治疗。该试纸条将基于一种能够选择性氧化GHB而不是乙醇的酶，以产生一种有色的、易于检测的产品。乙醇通常存在于GHB过量患者的体液中，因此这种测试也不应该与乙醇反应，因为会导致假阳性。 这项工作的另一个目标是开发一种测试条，不仅能够检测GHB，而且还能够检测同样有效的类似物γ-丁内酯（GBL）和1，4-丁二醇（BD）。这些GHB类似物在许多州是法律的，其滥用频率与GHB相当。它们在体内迅速代谢为GHB，因此同样危险。这种测试条将面向消费者销售，让他们在成为性侵犯受害者之前快速，轻松和谨慎地测试他们的饮料中的这些化合物。 这一快速通道提案的第一阶段目标是筛选我们广泛的商业酶系列对GHB、GBL和BD的活性，并构建和评估原型GHB检测试纸条。第二阶段将集中于大量生产伽马-羟丁酸测试条，并评估批间和批内的再现性和储存稳定性。第二阶段的另一个目标是为个人消费者开发一种多传感GHBGBL-BD检测条。后一种试纸也将以多个批次生产，并将确定重现性和储存稳定性。

DESCRIPTION (provided by applicant): Abuse of gamma-hydroxybutyrate (GHB) as a recreational drug is becoming increasingly more prevalent due to the general feeling of euphoria it produces when ingested in moderate levels. At higher levels, unconsciousness and coma can result making this the drug of choice in "date-rape" crimes. Because of this, the United States Drug Enforcement Administration has declared it a Schedule I Controlled Substance. The goal of this proposal is to create a rapid, accurate, and simple-to-use test strip capable of detecting GHB in bodily fluids. Such a test strip does not currently exist and therefore it is difficult for emergency room personnel and emergency service providers to tailor their treatment of intoxicated patients when the nature of the intoxicant is not known. The test strip will be based on an enzyme that is capable of selectively oxidizing GHB, but not ethanol, to produce a colored, easily detectable product. Ethanol is commonly found in bodily fluids of GHB overdose patients and therefore a test such as this should not also react with ethanol as false positives will result. An additional goal of this work will be to develop a test strip capable of detecting not only GHB but also the just as potent analogs gamma-butyrolactone (GBL) and 1,4-butanediol (BD). These GHB analogs are legal in many states and their frequency of abuse rivals that of GHB. They are quickly metabolized to GHB in vivo and therefore are just as dangerous. This test strip will be marketed towards consumers to allow them to quickly, easily and discreetly test their beverage for these compounds before they become a victim of sexual assault. The Phase I goals of this Fast-Track proposal will be to screen our extensive lines of commercial enzymes for activity towards GHB, GBL and BD, and to construct and evaluate a prototype GHB-detection test strip. Phase II will concentrate on mass producing the GHB test strip, and evaluating inter- and intra- batch reproducibility and storage stability. An additional goal of Phase II is to develop a multiple sensing GHBGBL- BD detection strip for individual consumers. The latter test strip will also produced in multiple batches and reproducibility and storage stability will be ascertained.