Development of rapid detection tests for B pseudomallei

假鼻疽杆菌快速检测方法的开发

• 批准号: 7116475
• 负责人: Raymond Houghton
• 金额: $ 54.13万
• 依托单位: INBIOS INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116475
• 关键词: Burkholderia; SDS polyacrylamide gel electrophoresis; bacterial antigens; bioinformatics; bioterrorism /chemical warfare; clinical research; diagnosis design /evaluation; diagnosis quality /standard; early diagnosis; enzyme linked immunosorbent assay; human tissue; immune complex; immunologic assay /test; laboratory mouse; microorganism classification; monoclonal antibody; rapid diagnosis; serotyping; systematic biology; western blottings

DESCRIPTION (provided by applicant): The Gram negative bacterium Burkholderia pseudomallei causes human melioidosis, a rapidly progressing and frequently fatal disease if not treated early. It is endemic in areas of NE Thailand, Southeast Asia and northern parts of Australia and is listed as a Category B agent of potential bioterrorist threats. Antibiotic treatment is difficult and prolonged with significant relapse rates and there is currently no vaccine available for protection of naturally or intentionally exposed individuals. Accurate tests for the rapid detection of this organism in blood samples and other clinical materials are needed to guide the critical early phases of clinical management but are not currently available. It is the purpose of this application to identify antigens specific to B. pseudomallei and use these to generate monoclonal antibodies for inclusion in a rapid field test for antigen detection particularly in acute infections. In addition, these antigens can be used directly to develop specific immunoblots for evaluating antibody responses in acute and chronic phases of disease. To achieve these goals, InBios (experienced in commercial development of rapid diagnostic assays) has brought together a team of experts in the UK with access to an animal model system for melioidosis with a group in Northeastern Thailand with access to clinical samples enabling Phase 1 field testing of the prototype assays produced. These tools will be invaluable in detecting and monitoring acute and chronic B. pseudomallei infections both in endemic areas as well as following a bioterrorist attack.

描述（由申请人提供）：革兰氏阴性细菌burkholderia pseudomallei会引起人类杂胞病，如果不早点治疗，迅速发展和经常致命的疾病。它在泰国东北，东南亚和澳大利亚北部地区是地方性的，被列为潜在生物恐怖威胁的B类代理商。抗生素治疗很困难，并且长时间延长了复发率，目前尚无疫苗可保护自然或有意暴露的个体。需要在血液样本和其他临床材料中快速检测该生物的准确测试，以指导临床管理的关键早期阶段，但目前尚不可用。该应用的目的是识别针对假单胞菌的抗原，并使用它们来生成单克隆抗体，以纳入抗原检测的快速现场测试中，尤其是在急性感染中。此外，这些抗原可直接用于开发特定的免疫印迹，以评估疾病急性和慢性阶段中的抗体反应。为了实现这些目标，INBIO（在快速诊断测定的商业开发方面经验丰富）将英国的专家团队汇集在一起​​，可以使用泰国东北部的一组梅尔利尼他的动物模型系统，并获得了临床样本，从而可以允许生产的原型测定期1阶段现场测试。这些工具在检测和监测急性和慢性B.假单胞菌感染以及遵循生物恐怖主义攻击的急性B.假单胞菌感染方面都是无价的。