IN VIVO PROTON MRS REVEALS BRAIN REGION SPECIFIC METABOLIC RESPONSES TO SIV

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 背景资料：对HIV感染者的体内质子磁共振波谱（1H-MRS）研究表明，不同脑区存在显著的代谢异常，但其原因尚不清楚。用磁共振波谱（MRS）研究了18只SIV感染猕猴在感染后1个月内额叶皮质、基底节和白色物质的代谢变化。 结果如下：N-乙酰天冬氨酸（NAA），胆碱（Cho），肌醇（MI），肌酸（Cr）和谷氨酰胺/谷氨酸（Glx）共振的变化进行了定量的绝对值和相对于肌酸共振。大多数异常是在病毒血症高峰期，感染后2周（wpi）时观察到的。在该时间点，仅在额叶皮层中观察到反映神经元损伤的NAA和NAA/Cr的显著降低。Cr仅在白色物质中显著升高。Cho和Cho/Cr的变化在整个脑区相似，在2 wpi时增加，在4 wpi时低于基线水平。所有脑区的MI和MI/Cr水平均升高。 结论：这些数据最好地支持这一假设，即不同的大脑区域对艾滋病病毒引起的神经元损伤具有不同的内在脆弱性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background: In vivo proton magnetic resonance spectroscopy (1H-MRS) studies of HIV-infected humans have demonstrated significant metabolic abnormalities that vary by brain region, but the causes are poorly understood. Metabolic changes in the frontal cortex, basal ganglia and white matter in 18 SIV-infected macaques were investigated using MRS during the first month of infection. Results: Changes in the N-acetylaspartate (NAA), choline (Cho), myo-inositol (MI), creatine (Cr) and glutamine/glutamate (Glx) resonances were quantified both in absolute terms and relative to the creatine resonance. Most abnormalities were observed at the time of peak viremia, 2 weeks post infection (wpi). At that time point, significant decreases in NAA and NAA/Cr, reflecting neuronal injury, were observed only in the frontal cortex. Cr was significantly elevated only in the white matter. Changes in Cho and Cho/Cr were similar across the brain regions, increasing at 2 wpi, and falling below baseline levels at 4 wpi. MI and MI/Cr levels were increased across all brain regions. Conclusion: These data best support the hypothesis that different brain regions have variable intrinsic vulnerabilities to neuronal injury caused by the AIDS virus.