POTENTIAL FOR TRANSMISSION OF BLOOD BORNE PATHOGENS BY SYRINGES IN CAMEROON

喀麦隆通过注射器传播血源性病原体的可能性

• 批准号: 7349045
• 负责人: CRISTIAN APETREI
• 金额: $ 6.54万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349045
• 关键词: POTENTIAL; TRANSMISSION; BLOOD; BORNE; PATHOGENS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Viral contamination of clinical injections in Kumba, Cameroon was examined in 1536 patients in 2001¿2002. Blood and matched syringe washes (SW) were collected from each patient. Antibodies against HIV, HBV and HCV were identified in blood and SW. Seroprevalence was 12.4% for HIV, 10.75% for HBsAg and 10.12% for HCV. HIV RNA was extracted from positive and indeterminate washings. gag, pol and env genes were amplified from 32% of intravenous injections and 2% of intramuscular injections Thirty-six HIV-1 group M strains were partially sequenced. They majority belonged to the circulating recombinant form (CRF)02(IbNg). Subtypes A, D, F2 and CRF11_cpx were also present at less than 3% each. These findings support a role for unsafe injections in the spread of HIV-1 and other viruses in West Central Africa and are in contrast to recent studies indicating that contamination of injection equipment was not possible in these settings.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。 2001 年至 2002 年，喀麦隆昆巴对 1536 名患者进行了临床注射病毒污染检查。从每位患者身上收集血液和匹配的注射器清洗液（SW）。在血液和 SW 中鉴定出抗 HIV、HBV 和 HCV 的抗体。 HIV 血清阳性率为 12.4%，HBsAg 为 10.75%，HCV 为 10.12%。从阳性和不确定的洗涤液中提取 HIV RNA。 32% 的静脉注射和 2% 的肌肉注射中扩增了 gag、pol 和 env 基因。 对 36 个 HIV-1 M 组毒株进行了部分测序。它们大多数属于循环重组形式 (CRF)02(IbNg)。亚型 A、D、F2 和 CRF11_cpx 的含量也均低于 3%。这些发现支持了不安全注射在中非西部地区 HIV-1 和其他病毒传播中的作用，并且与最近的研究形成鲜明对比，这些研究表明在这些环境中注射设备不可能受到污染。