POTENTIAL FOR TRANSMISSION OF BLOOD BORNE PATHOGENS BY SYRINGES IN CAMEROON

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。2001年至2002年，在喀麦隆昆巴对1536名患者进行了临床注射的病毒污染检查。从每个患者身上采集血液和匹配的注射器洗涤剂(Sw)。血和血中均检出抗HIV、抗-HBs和抗-抗-HCV抗体。HIV抗体阳性率为12.4%，乙肝表面抗原阳性率为10.75%，丙型肝炎病毒阳性率为10.12%。从阳性和不确定的洗涤液中提取HIV RNA。从32%的静脉注射和2%的肌肉注射中扩增出Gag、Pol1和env基因。36株HIV-1 M组毒株被部分测序。它们大多属于循环重组形式(CRF)02(IbNg)。A亚型、D亚型、F2亚型和CRF11_cpx亚型也分别低于3%。这些发现支持不安全注射在中非西部艾滋病毒-1和其他病毒传播中的作用，并与最近的研究形成对比，这些研究表明，在这些环境中不可能污染注射设备。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Viral contamination of clinical injections in Kumba, Cameroon was examined in 1536 patients in 2001¿2002. Blood and matched syringe washes (SW) were collected from each patient. Antibodies against HIV, HBV and HCV were identified in blood and SW. Seroprevalence was 12.4% for HIV, 10.75% for HBsAg and 10.12% for HCV. HIV RNA was extracted from positive and indeterminate washings. gag, pol and env genes were amplified from 32% of intravenous injections and 2% of intramuscular injections Thirty-six HIV-1 group M strains were partially sequenced. They majority belonged to the circulating recombinant form (CRF)02(IbNg). Subtypes A, D, F2 and CRF11_cpx were also present at less than 3% each. These findings support a role for unsafe injections in the spread of HIV-1 and other viruses in West Central Africa and are in contrast to recent studies indicating that contamination of injection equipment was not possible in these settings.