RBC Ion Transporters as Hemoglobinopathy Risk Modifiers

红细胞离子转运蛋白作为血红蛋白病风险调节剂

基本信息

  • 批准号:
    7245127
  • 负责人:
  • 金额:
    $ 39.02万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2006
  • 资助国家:
    美国
  • 起止时间:
    2006-06-15 至 2010-05-31
  • 项目状态:
    已结题

项目摘要

Description (provided by applicant): Sickle cell disease and thalassemia are characterized by pathological red cell dehydration. The mean corpuscular hemoglobin concentration of sickle erythrocytes critically determines the lag time preceding the rapid phase of deoxygenation-induced polymerization of hemoglobin S. Several erythroid ion transporters and channels are believed on the basis of pharmacological and physiological studies to regulate red cell hemoglobin concentration secondary to regulation of red cell volume. Among these activities already studied as therapeutic targets in sickle cell disease are the KCNN/IK1/SK4 Ca2+-activated K+ channel of intermediate conductance (Gardos channel), several types of KCC K-CI cotransporters, at least two types of erythroid CI- conductance, and at least one type of Ca2+permeable cation conductance. The K-CI cotransporters have also been tested as therapeutic targets for thalassemia. The genes encoding these ion-transporting polypeptides are strong candidate risk modifier genes for the hemoglobinopathies. This application proposes the general hypothesis that genetic modulation of these transporter and channel activities will modulate disease severity in mouse models of hemoglobinopathies. This general hypothesis will be tested by experiments designed to pursue the following Specific Aims: Aim 1. We will test the hypothesis that genetic deficiency of the erythroid IK1/Gardos channel will decrease pathologic red cell dehydration and will ameliorate clinical severity in mouse models of sickle cell disease. Aim 2. We will test the hypothesis that genetic deficiency of erythroid KCC K-CI cotransporters will decrease pathologic red cell dehydration and will ameliorate clinical severity in mouse models of sickle cell disease and of beta-thalassemia intermedia. Aim 3. We will test the hypothesis that combined genetic deficiency of erythroid IK1/Gardos channel and of erythroid KCC K-CI cotransporters will further ameliorate clinical severity in mouse models of sickle cell disease. The proposed experiments will increase understanding of sickle cell disease and thalassemia by providing mouse models for genetic tests of new drug therapies under development for near-term clinical testing.
描述(由申请方提供):镰状细胞病和地中海贫血的特征是病理性红细胞脱水。镰状红细胞的平均红细胞血红蛋白浓度决定了脱氧诱导的血红蛋白S聚合的快速阶段之前的滞后时间。基于药理学和生理学研究,认为几种红细胞离子转运蛋白和通道调节红细胞血红蛋白浓度,其次是调节红细胞体积。在镰状细胞病中作为治疗靶点已经研究的这些活性中有中等电导的KCNN/IK 1/SK 4 Ca 2+激活的K+通道(Gardos通道)、几种类型的KCC K-Cl共转运蛋白、至少两种类型的红细胞Cl-电导和至少一种类型的Ca 2+渗透性阳离子电导。K-CI协同转运蛋白也已被测试为地中海贫血的治疗靶标。编码这些离子转运多肽的基因是血红蛋白病的强有力的候选风险调节基因。本申请提出了一般假设,即这些转运蛋白和通道活性的遗传调节将调节血红蛋白病小鼠模型中的疾病严重程度。这一一般假设将通过旨在追求以下具体目标的实验进行检验:目标1。我们将检验红系IK 1/Gardos通道的遗传缺陷将减少病理性红细胞脱水并改善镰状细胞病小鼠模型的临床严重程度的假设。目标二。我们将测试红系KCC K-CI协同转运蛋白的遗传缺陷将减少病理性红细胞脱水并将改善镰状细胞病和中间型β地中海贫血小鼠模型的临床严重程度的假设。目标3.我们将检验红系IK 1/Gardos通道和红系KCC K-CI协同转运蛋白的联合遗传缺陷将进一步改善镰状细胞病小鼠模型的临床严重程度的假设。拟议的实验将通过为正在开发的用于近期临床测试的新药物疗法的基因测试提供小鼠模型,增加对镰状细胞病和地中海贫血的了解。

项目成果

期刊论文数量(0)
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SETH Leo ALPER其他文献

SETH Leo ALPER的其他文献

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{{ truncateString('SETH Leo ALPER', 18)}}的其他基金

Molecular Mechanism of APOL1 Associated Kidney Disease
APOL1相关肾脏疾病的分子机制
  • 批准号:
    8486603
  • 财政年份:
    2013
  • 资助金额:
    $ 39.02万
  • 项目类别:
Molecular Mechanism of APOL1 Associated Kidney Disease
APOL1相关肾脏疾病的分子机制
  • 批准号:
    8791547
  • 财政年份:
    2013
  • 资助金额:
    $ 39.02万
  • 项目类别:
Molecular Mechanism of APOL1 Associated Kidney Disease
APOL1相关肾脏疾病的分子机制
  • 批准号:
    9011946
  • 财政年份:
    2013
  • 资助金额:
    $ 39.02万
  • 项目类别:
Molecular Mechanism of APOL1 Associated Kidney Disease
APOL1相关肾脏疾病的分子机制
  • 批准号:
    9212011
  • 财政年份:
    2013
  • 资助金额:
    $ 39.02万
  • 项目类别:
Molecular Mechanism of APOL1 Associated Kidney Disease
APOL1相关肾脏疾病的分子机制
  • 批准号:
    8695481
  • 财政年份:
    2013
  • 资助金额:
    $ 39.02万
  • 项目类别:
RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
红细胞离子转运蛋白作为血红蛋白病风险调节剂
  • 批准号:
    7030496
  • 财政年份:
    2006
  • 资助金额:
    $ 39.02万
  • 项目类别:
RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
红细胞离子转运蛋白作为血红蛋白病风险调节剂
  • 批准号:
    7629010
  • 财政年份:
    2006
  • 资助金额:
    $ 39.02万
  • 项目类别:
RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
红细胞离子转运蛋白作为血红蛋白病风险调节剂
  • 批准号:
    7435221
  • 财政年份:
    2006
  • 资助金额:
    $ 39.02万
  • 项目类别:
RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
红细胞离子转运蛋白作为血红蛋白病风险调节剂
  • 批准号:
    7459154
  • 财政年份:
    2006
  • 资助金额:
    $ 39.02万
  • 项目类别:
RBC Ion Transporters as Hemoglobinopathy Risk Modifiers
红细胞离子转运蛋白作为血红蛋白病风险调节剂
  • 批准号:
    7665605
  • 财政年份:
    2006
  • 资助金额:
    $ 39.02万
  • 项目类别:

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  • 批准号:
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  • 财政年份:
    1960
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