Biophysical mechanisms of stem cell regulation

干细胞调节的生物物理机制

• 批准号: 7222858
• 负责人: Nestor J Oviedo
• 金额: $ 4.96万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7222858
• 关键词: Address; Adult; Area; Attention; Behavior; Biochemical; Biological; Biological Markers; Biological Models; Cell Cycle; Cells; Cellular biology; Communication; Complement; Complex; Computer information processing; Cues; Daily; Data; Developmental Biology; Elements; Embryo; Event; Future; Genes; Genomics; Goals; Homeostasis; Human body; Injury; Invertebrates; Ion Channel; Ions; Malignant Neoplasms; Mediating; Modeling; Molecular; Molecular Genetics; Morphogenesis; Morphology; Natural regeneration; Organism; Phenotype; Physiological; Planarians; Platyhelminths; Population; Process; Proliferating; Publications; Pump; RNA Interference; Range; Regulation; Research; Retinal Cone; Role; Scientist; Signal Transduction; Source; Staging; Stem cells; Systems Biology; Testing; Tissues; Training; Transcript; Undifferentiated; Validation; Work; adult stem cell; base; cell behavior; cell growth regulation; design; experience; insight; loss of function; migration; novel; pH gradient; research study; restoration; senescence; tissue regeneration; voltage

DESCRIPTION (provided by applicant): The interplay between undifferentiated and differentiated tissue allows the fine regulation of body homeostasis. Much is unknown about the molecular events underlying this process, but it is likely to involved adult stem cell activation. In addition to homeostasis, stem cells may be harnessed to induce the regeneration of tissue damaged by senescence, cancer, or injury. Such biomedical approaches require a basic molecular understanding of stem cells in their natural behavior. In complement to the current focus on biochemical signals, we will explore an important but largely mysterious set of cellular control parameters: ion flows. The main hypothesis that will be tested in this proposal is that stem cells are controlled in part by biophysical signals mediated by gap junctional communication and the activity of ion channels and pumps. Capitalizing on a powerful model system, the planarian, I propose to gain molecular, mechanistic insight into the novel and important role of endogenous physiological signals regulating the interactions between adult stem cells and differentiating tissue during physiological turnover.and regeneration.

描述（由申请人提供）：未分化组织和分化组织之间的相互作用允许身体稳态的精细调节。这一过程背后的分子机制尚不清楚，但很可能与成体干细胞活化有关。除了体内平衡，干细胞还可用于诱导因衰老、癌症或损伤而受损的组织再生。这种生物医学方法需要对干细胞的自然行为有基本的分子理解。为了补充目前对生化信号的关注，我们将探索一组重要但很大程度上神秘的细胞控制参数：离子流。在本提案中将测试的主要假设是，干细胞部分地由间隙连接通信介导的生物物理信号以及离子通道和泵的活性控制。利用一个强大的模型系统，涡虫，我建议获得分子，机制洞察内源性生理信号调节成体干细胞和分化组织之间的相互作用的新的和重要的作用，在生理周转和再生。