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The Effects of Direct Current Stimulation in Adult Tissues

直流电刺激对成人组织的影响

基本信息

批准号：
10380856
负责人：
Nestor J Oviedo
金额：
$ 30.74万
依托单位：
UNIVERSITY OF CALIFORNIA, MERCED
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-05-16 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10380856
关键词：
Address Adopted Adult Affect Alzheimer&apos s Disease Behavior Biological Biological Models Biophysics Body Regions Body part Calcium Signaling Cell Cycle Cell Death Cell Differentiation process Cell Proliferation Cells Clinical Cognition Coin Complex Cues DNA Repair Disease Early Gene Transcriptions Electric Stimulation Electrophysiology (science)Equipment Exhibits Genetic Genetic Transcription Genomics Human Ice Immediate-Early Genes Injury Instruction Intervention Invertebrates Knowledge Lead Measures Mediating Mediator of activation protein Mental Depression Methodology Methods Migraine Mitosis Mitotic Modality Molecular Molecular Genetics Monitor Muscle Cells Natural regeneration Neurologic Organism Parkinson Disease Patients Pattern Pharmacological Treatment Pharmacology Pharmacology Study Pharyngeal structure Photoreceptors Physiologic pulse Physiological Planarians Platyhelminths Process Protein Biosynthesis Psyche structure Radiation therapy Recovery Regulation Research Signal Pathway Signal Transduction Specific qualifier value Spectrum Analysis Stimulus Testing Time Tissue Transplantation Tissues Transcriptional Activation Transcriptional Regulation Translating Transplantation WNT Signaling Pathway Whole Organism base bone repair cell behavior cell type cellular targeting direct application genetic information improved in vivo insight interdisciplinary approach millisecond multitask nervous system disorder novel novel strategies prevent reconstitution release of sequestered calcium ion into cytoplasm replacement tissue response skills stem cell biomarkers stem cell differentiation stem cell division stem cell migration stem cell proliferation stem cells tissue regeneration tool treatment duration

项目摘要

Project Summary/Abstract The exogenous application of electric currents is an effective method widely used to treat a plethora of clinical conditions and to enhance mental skills including multitasking capability and numerical cognition in healthy humans. Despite the wide use of localized direct current stimulation (DCS) in experimental, clinical and public settings, the molecular bases of its effects in various cell types are unknown. We seek to gain fundamental knowledge about the effects and signals mediating DCS in adult tissues. Therefore, we focus on basic electrophysiology research using a model system based on planarian flatworms, which is amenable to study various DCS modalities and their effects on tissues at systemic and cellular levels. We plan to merge molecular genetics and electrophysiology to define instructive mechanisms underlying cellular responses to DCS. Planarians activate stem cells (SCs) called neoblasts to continually renew adult tissues and are capable of regenerating any part of their body upon injury. Our research team developed a novel strategy based on the external application of DCS of physiological strength to the whole planarian body. We provide compelling evidence that it is possible to use DCS similar to the one used in humans to control collective neoblast behavior in vivo (i.e. regulation of transcription, cell cycle, cell death, differentiation), and induce permanent changes in adult tissue identity without using genetic or pharmacological treatments. The integration of biophysical and genetic information may provide a paradigm shift in the way we approach SC regulation in the adult body and by executing this project we expect to uncover mechanistic insights about this process. A major impact of these studies will be the elucidation of basic mechanisms mediating DCS effects in the complexity of the whole organism to enable the implementation of biomedical approaches to induce selective tissue replacements and control cellular behavior to prevent or treat disease.

项目总结/摘要外源性施加电流是一种广泛用于治疗多种临床疾病的有效方法。条件和提高心理技能，包括多任务处理能力和数字认知，人类尽管局部直流电刺激（DCS）在实验、临床和公众中被广泛使用，在这种情况下，它在各种细胞类型中的作用的分子基础是未知的。我们力求从根本上了解成人组织中DCS的作用和信号。因此，我们专注于基础电生理学研究使用的模型系统的基础上，Planarian扁形虫，这是适合研究各种DCS模式及其在全身和细胞水平对组织的影响。我们计划合并分子遗传学和电生理学，以确定细胞反应的指导机制，区议会Planarians激活称为neoblast的干细胞（SC），以不断更新成体组织，在受伤后再生身体的任何部分。我们的研究团队开发了一种基于将生理强度的DCS外敷于整个涡虫体。我们提供令人信服的有证据表明，有可能使用类似于人类使用的DCS来控制集体新生细胞。在体内的行为（即转录，细胞周期，细胞死亡，分化的调节），并诱导永久性在不使用遗传或药物治疗的情况下改变成人组织特性。的整合生物物理学和遗传学信息可能为我们处理SC调节的方式提供范式转变，通过执行这个项目，我们希望揭示有关这一过程的机械见解。一个主要这些研究的影响将是阐明介导DCS效应的基本机制，整个生物体，以使生物医学方法的实施，替代和控制细胞行为来预防或治疗疾病。