The Role of Wnt2 in Drosophila Antenna Lobe Development

Wnt2 在果蝇触角发育中的作用

• 批准号: 7275613
• 负责人: FAITH L LIEBL
• 金额: $ 3.38万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-16 至 2007-08-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7275613
• 关键词: Adult; Animals; Antibodies; Axon; Brain; Cell physiology; Cells; Chromosome Pairing; Commissure; Data; Dendrites; Development; Drosophila genus; Exhibits; Family; Genes; Green Fluorescent Proteins; In Situ Hybridization; Insecta; Label; Lobe; Mammals; Maps; Maxilla; Membrane; Molecular; Neuroglia; Neurons; Odorant Receptors; Odors; Olfactory Receptor Neurons; Organ; Pattern; Perception; Peripheral; Play; Population; Process; Protein Overexpression; Proteins; Receptor Gene; Role; Sensory; Signal Transduction; Source; Specific qualifier value; Staging; Structure; Synapses; Techniques; Testing; axon guidance; cell type; human WNT2 protein; knock-down; mature animal; member; mutant; nerve supply; olfactory bulb; olfactory lobe; postsynaptic; presynaptic; proto-oncogene int-1 related protein; research study; vesicle-associated membrane protein

DESCRIPTION (provided by applicant): The perception of odors requires olfactory information to be systematically organized in the brain. This organization, termed the olfactory map, consists of precise connections between olfactory receptor neurons and their postsynaptic targets. The molecular mechanisms responsible for olfactory map formation, however, is not well understood. I have discovered that Wnt2, a member of the conserved Wnt family of secreted proteins, is necessary for the organization of the Drosophila AL, an olfactory structure composed of olfactory receptor neurons (ORNs) that synapse upon projection neurons. In each All, axons expressing a given odorant receptor gene converge on a single glomerulus. The wnt2 gene plays a powerful role in specifying the precise connectivity of the Drosophila olfactory map by organizing the AL. Animals lacking a functional wnt2 gene exhibit severely disorganized, smaller ALs. Labeling of specific ORNs reveals that a large proportion of ORN axons are missing from the ALs and the structures of the glomeruli are severely degraded. Overexpression of Wnt2 induces the formation of ectopic synapses in the AL commissure, a structure normally composed of tracts of axons. I hypothesize that Wnt2 regulates both olfactory receptor neuron axon guidance and synaptic differentiation within the antenna lobe. The aim of this proposal is to investigate the role of Wnt2 in olfactory map formation by determining its cellular function, developmental requirements, and the cell types that express Wnt2.

描述（由申请人提供）：气味的感知需要嗅觉信息在大脑中系统地组织起来。这个组织，被称为嗅觉地图，由嗅觉受体神经元和它们的突触后靶点之间的精确连接组成。然而，负责嗅觉地图形成的分子机制还没有很好地理解。我已经发现，Wnt 2，一个保守的Wnt家族成员的分泌蛋白，是必要的组织果蝇AL，嗅觉结构组成的嗅觉受体神经元（ORN），突触投射神经元。在每一个细胞中，表达特定气味受体基因的轴突聚集在单个肾小球上。wnt 2基因在指定果蝇嗅觉地图的精确连接中起着重要作用，通过组织AL。缺乏功能性wnt 2基因的动物表现出严重混乱的，较小的AL。特异性ORN的标记揭示了AL中大部分ORN轴突缺失，肾小球结构严重退化。Wnt 2的过表达诱导AL连合中异位突触的形成，AL连合是通常由轴突束组成的结构。我推测，Wnt 2调节嗅觉受体神经元轴突的指导和突触分化的天线叶内。该提案的目的是通过确定其细胞功能，发育要求和表达Wnt 2的细胞类型来研究Wnt 2在嗅觉地图形成中的作用。