Axon branching during auditory circuit assembly

听觉回路组装过程中的轴突分支

• 批准号: 7214104
• 负责人: CINDY C LU
• 金额: $ 4.88万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7214104
• 关键词: Auditory; Axon; Behavior; Cells; Cochlea; Cochlear nucleus; Coculture Techniques; Complex; Cues; Development; Dorsal; Environment; Event; Fluorescence; Ganglia; Genetic; Genetic Techniques; Growth Cones; Hair Cells; Hearing; Image; In Vitro; Individual; Label; Life; Location; Mus; Myxoid cyst; Nature; Neurons; Population; Property; Relative (related person); Reporter; Staging; System; Tamoxifen; Techniques; Time; Tissues; Vestibular ganglion; axon growth; axon regeneration; hindbrain; insight; novel strategies; response; sound; stimulus processing

DESCRIPTION (provided by applicant): Functional hearing depends on the formation of appropriate connections between neurons in the auditory circuit during development. Axons of cochlear ganglion neurons bifurcate upon reaching the hindbrain in order to distribute information from cochlear hair cells to both divisions of the cochlear nucleus. There, the tonotopic organization of the cochlea is preserved and different features of complex sound stimuli are processed separately. In order to determine the timing and location of axon branching, cochlear ganglion projections will be visualized using genetic techniques in the mouse. Individual projections will be labeled in order to define the sequence of events that occur during branch formation at the single cell level. Further insights into the cellular mechanisms of axon branching will come from live imaging of individual cochlear ganglion axons. To begin to define the relative contributions of intrinsic and extrinsic factors, cochlear and vestibular neurons will be compared for their abilities to branch in isolation or when co-cultured with hindbrain tissue. These studies will contribute significantly to our understanding of the assembly and function of auditory circuits and may reveal novel strategies to promote axon regeneration.

描述（申请人提供）：功能性听力取决于发育过程中听觉回路中神经元之间形成适当的连接。耳蜗神经节神经元轴突在到达后脑后分叉，目的是将耳蜗毛细胞的信息传递给耳蜗核的两个分裂。在那里，保留耳蜗的张力组织，并分别处理复杂声刺激的不同特征。为了确定轴突分支的时间和位置，将使用遗传技术在小鼠中可视化耳蜗神经节的投影。为了确定在单细胞水平上分支形成过程中发生的事件序列，将对单个突起进行标记。进一步了解轴突分支的细胞机制将来自于单个耳蜗神经节轴突的实时成像。为了开始定义内在和外在因素的相对贡献，将比较耳蜗和前庭神经元在分离或与后脑组织共培养时的分支能力。这些研究将有助于我们对听觉回路的组装和功能的理解，并可能揭示促进轴突再生的新策略。