Robotics Extraction System/HPLC for Shared Resource Lab

描述(由申请人提供)：拨款申请申请资金用于购买Gilson SPE 215自动液体处理和固相萃取/高压层析系统，以促进维克森林大学医学院高血压中心分析核心实验室对生物样本的处理和分析。目前，所有样本处理和提取都是由高血压核心人员在布里奇特·布罗斯尼汉博士的监督下手动进行的。鉴于在分析之前需要大量的样本，以及对各种组织、血液、尿液和细胞培养中不同的RIA或ELISA法的需求不断增加，因此需要一个自动化的处理和提取系统来保持高血压核心实验室的进度、效率和产量。此外，所要求的仪器允许将提取的样品直接注入高效液相色谱系统(HPLC)以进行样品和化验验证。虽然不是对每个样本进行常规检查，但无论是单个样本还是混合样本，在进行免疫学分析时，特别是在结合广泛的提取方法时，都能确保样本的一致性。这一核心设施为15名NIH资助的调查人员提供支持，这些调查人员持有17项NIH赠款和一项具有5个单独项目的P01；WFUSM的调查人员提交的需要这种设备的样本中，超过90%是由NIH资助的。高血压核心实验室在方便的校园环境中为研究人员提供了一种低成本的多分析设施。最先进的采样/提取/高效液相色谱系统的加入将使高血压核心实验室能够满足不断增加的样本负载，并减少向研究人员输出数据的时间。因此，高质量的仪器设备、非常有经验的人员和坚实的财政支持相结合，将确保所有由NIH资助的研究人员在收购拟议仪器设备后在时间、数量和质量上都能受益。

DESCRIPTION (provided by applicant): The grant application requests funds for the purchase of a Gilson SPE 215 automated liquid handling and solid phase extraction/high pressure chromatography system to facilitate the processing and analysis of biological samples by the Hypertension Center Assay Core laboratory at the Wake Forest University School of Medicine (WFUSM). Currently all sample handling and extractions are performed manually by the Hypertension Core personnel, under the supervision of Dr. Bridget Brosnihan. Given the large amount of samples and increasing demand for different RIA or ELISA procedures in various tissues, blood, urine and cell cultures that all require extensive extraction procedures prior to analysis, an automated handling and extraction system is necessary to maintain the precession, efficiency and output of the Hypertension Core Laboratory. Furthermore, the requested instrument allows for direct injection of extracted samples into a high performance liquid chromatography system (HPLC) for sample and assay validation. Although not routinely performed for each sample, HPLC verification of either individual or pooled samples ensures sample identity in immunological analysis performed particularly when combined with extensive extraction methods. This core facility provides support for 15 NIH-funded investigators holding 17 NIH grants and a P01 with 5 individual projects; over 90% of the samples submitted by investigators at WFUSM requiring this equipment are funded by NIH. The Hypertension Core Laboratory offers a low cost, multi-assay facility to investigators in a convenient on-campus setting. The addition of the state-of-the-art sampling/extraction/HPLC system will allow the Hypertension Core Laboratory to meet the increasing sample load and reduce the delivery time of data output to investigators. Therefore, the combination of high quality instrumentation, very-experienced personnel and solid financial support will ensure all NIH-funded researchers will benefit in time, quantity and quality with the acquisition of the proposed instrumentation.