Acquisition of a MALDI-TOF Spectrometer

描述（由申请人提供）：寻求资助PE Biosystems Voyager DE PRO MALDI/TOF质谱仪。 MALDI/TOF质谱法是对肽、蛋白质和聚合物、非挥发性和/或热不稳定分子进行质量分析的首选方法。质谱仪将位于斯托尼布鲁克化学生物学与药物发现研究所的分析仪器设施。这将大大提高来自斯托尼布鲁克的七位知名研究科学家的研究生产力，他们是主要用户群体。NIH资助的研究探索了控制蛋白质生物传感器光谱特性的结构-功能关系，酶催化，蛋白质催化剂的形成，蛋白质折叠，癌症治疗，配体介导的信号转导和糖基化。MALDI/TOF对于这些需要肽合成、聚合物合成、蛋白质连接和糖肽图谱的项目至关重要。这些样品中的许多是合成制备的。在进行进一步实验之前，需要进行质谱分析以验证是否获得了正确的产物，并分析可能发生的副反应。因此，同一天的可用性是我们研究企业成功的关键。其他样品难以分离，需要方法开发。这种方法的开发需要了解所研究样品的性质，投入时间寻找最佳电离条件，并注意不要浪费有限数量的样品。该仪器将由化学生物学和药物发现分析仪器设施研究所的经过培训的人员操作和维护。设施主任将安排日常运作，并为用户提供技术专门知识。研究支持专家将为不需要“动手”分析自己样品的用户运行样品。化学生物学和药物发现指导委员会将监督仪器的管理，确保主要用户和其他NIH资助的研究人员的访问，并管理仪器的长期运行。斯托尼布鲁克管理局和化学生物学与药物发现研究所对仪器的维护和操作做出了重大贡献。

DESCRIPTION (provided by applicant): Funding is sought for a PE Biosystems Voyager DE PRO MALDI/TOF Mass Spectrometer. MALDI/TOF mass spectrometry is the method of choice for mass analysis of peptides, proteins, and polymers, molecules that are non-volatile and/or thermally unstable. The mass spectrometer will be located in the Analytical Instrumentation Facility of the Institute of Chemical Biology & Drug Discovery at Stony Brook. It will significantly enhance the research productivity of seven established research scientists from Stony Brook who comprise the major user group. The NIH-funded studies explore the structure-function relationships that control spectroscopic properties of protein biosensors, enzyme catalysis, formation of protein catalysts, protein folding, cancer therapy, ligand-mediated signal transduction, and glycosylation. MALDI/TOF is essential for these projects that require peptide synthesis, polymer synthesis, protein ligation, and glycopeptide mapping. Many of these samples are synthetically prepared. Mass spectral analyses are required to verify that the correct product has been obtained before proceeding with further experiments, and for analyzing side-reactions that may have occurred. Thus, same day availability is key to the success of our research enterprise. Other samples are difficult to ionize, and require method development. This method development requires a knowledge of the properties of the samples under investigation, an investment of time to find the best ionization conditions, and care to not waste limited amounts of samples. The instrument will be operated and maintained by trained personnel in the Institute of Chemical Biology & Drug Discovery Analytical Instrumentation Facility. The Director of the Facility will schedule daily operation and provide technical expertise for users. A Research Support Specialist will run samples for users who do not require "hands-on" analysis of their own samples. The Institute of Chemical Biology & Drug Discovery steering committee will oversee administration of the instrument, ensure access to major users and other NIH-funded investigators, and manage long term operation of the instrument. The Stony Brook administration and the Institute of Chemical Biology & Drug Discovery have made a significant contribution toward the maintenance and operation of the instrument.