Biacore 3000

描述(申请人提供)：来自费城儿童医院9个科室的10名研究人员正在研究各种对受体生物合成、细胞凋亡、蛋白质聚集、血细胞发育和血管生物学重要的蛋白质-蛋白质相互作用过程。所有这些研究项目的共同之处是需要对蛋白质-蛋白质和蛋白质-配体相互作用进行定量测量。因此，我们请求资金购买Biacore 3000系统，用于测量动力学结合参数。这种表面等离子体共振技术在许多应用中越来越成为一种选择的技术，因为与其他技术相比，它需要少量的纯化蛋白质。Biacore 3000将安装在我们新的蛋白质核心设施中，并将补充质谱仪、分析离心法和层析，为社区提供一套目前在校园中无法使用的蛋白质相互作用工具。Biacore 3000将在费城儿童医院的额外支持下进行充电。它要么是用户操作的系统，要么是全面服务的系统，这取决于用户的选择。该项目将由该中心主任M·罗森布拉特博士负责监督，并与指导委员会成员菲利普斯博士、伊斯罗波洛斯博士和克里希纳什瓦米博士合作。主要用户将占用大约75%的仪器时间，其余时间提供给费城儿童医院的所有研究人员。

DESCRIPTION (provided by applicant): A diverse group of 10 investigators from 9 divisions at the Children's Hospital of Philadelphia are studying a variety of protein-protein interaction processes important for receptor biosynthesis, apoptosis, protein aggregation, blood cell development and vascular biology. Common to all these research programs is the need for quantitative measurements of protein-protein and protein-ligand interactions. We therefore request funds to purchase a Biacore 3000 system for measurements of kinetic binding parameters. This surface plasmon resonance technology is increasingly a technique of choice in many applications, because it requires small quantities of purified proteins in comparison to other techniques. The Biacore 3000 will be housed in our new Protein Core Facility and will complement mass spectrometry, analytical centrifugation and chromatography, to provide the community with a suite of protein interaction tools, that are currently not available on campus. The Biacore 3000 will operate on a recharge basis with additional support from the Children's Hospital of Philadelphia. It will be either a user-operated or a full-service system, depending on a user's choice. It will be overseen by Dr. M. Rosenblatt, the facility's director, working with the PI and Drs. Phillips, Ischiropoulos and Krishnashwamy, who constitute the steering committee. The major users will occupy approximately 75% of the instrument time, with remaining time made available to all Children's Hospital of Philadelphia researchers.