Biacore 3000

描述（由申请人提供）：来自费城儿童医院9个部门的10名研究人员组成的多样化小组正在研究对受体生物合成、细胞凋亡、蛋白质聚集、血细胞发育和血管生物学重要的各种蛋白质-蛋白质相互作用过程。所有这些研究项目的共同点是需要定量测量蛋白质-蛋白质和蛋白质-配体相互作用。因此，我们要求资金购买Biacore 3000系统，用于测量动力学结合参数。这种表面等离子体共振技术越来越成为许多应用中的首选技术，因为与其他技术相比，它需要少量的纯化蛋白质。Biacore 3000将被安置在我们新的蛋白质核心设施，并将补充质谱，分析离心和色谱，为社区提供一套蛋白质相互作用的工具，目前在校园里还没有。Biacore 3000将在费城儿童医院的额外支持下充电运行。这将是一个由用户操作的系统或一个全面服务的系统，取决于用户的选择。它将由M博士监督。罗森布拉特，该机构的主任，与PI和博士。菲利普斯，Ijuropoulos和Krishnashwamy，谁构成指导委员会。主要用户将占用约75%的仪器时间，剩余时间提供给费城儿童医院的所有研究人员。

DESCRIPTION (provided by applicant): A diverse group of 10 investigators from 9 divisions at the Children's Hospital of Philadelphia are studying a variety of protein-protein interaction processes important for receptor biosynthesis, apoptosis, protein aggregation, blood cell development and vascular biology. Common to all these research programs is the need for quantitative measurements of protein-protein and protein-ligand interactions. We therefore request funds to purchase a Biacore 3000 system for measurements of kinetic binding parameters. This surface plasmon resonance technology is increasingly a technique of choice in many applications, because it requires small quantities of purified proteins in comparison to other techniques. The Biacore 3000 will be housed in our new Protein Core Facility and will complement mass spectrometry, analytical centrifugation and chromatography, to provide the community with a suite of protein interaction tools, that are currently not available on campus. The Biacore 3000 will operate on a recharge basis with additional support from the Children's Hospital of Philadelphia. It will be either a user-operated or a full-service system, depending on a user's choice. It will be overseen by Dr. M. Rosenblatt, the facility's director, working with the PI and Drs. Phillips, Ischiropoulos and Krishnashwamy, who constitute the steering committee. The major users will occupy approximately 75% of the instrument time, with remaining time made available to all Children's Hospital of Philadelphia researchers.