PROTEOMICS/OXIDATIVE STRESS CORE

蛋白质组学/氧化应激核心

• 批准号: 6946254
• 负责人: HOLLY VAN REMMEN
• 金额: $ 8.26万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6946254
• 关键词: arachidonate; biomedical facility; deoxyguanosine; electrochemistry; electrospray ionization mass spectrometry; fluorescent dye /probe; high performance liquid chromatography; matrix assisted laser desorption ionization; oxidative stress; proteomics

The goal of the Proteomics/Oxidative Stress Core is two-fold. First, we will provide investigators with the equipment and technical assistance for the identification of newly discovered proteins from various tissue and cellular compartments and analysis of alterations in protein levels. Analysts and discovery of age-associated proteins in specific cellular compartments will potentially provide important new insights into pathways or metabolic processes integral to the aging process. As a second function, we will measure oxidative damage to lipids and DNA in cells and tissues using the latest and most sensitive methods and technologic approaches. Accumulation of oxidative damage to cell components with age has been proposed as a primary factor responsible for age associated alterations in physiology and pathology. Thus, it is essential that endpoints of oxidative damage be measured accurately. The measurements described above require costly equipment and technologic expertise that prevent these types of analysis as routine measures in individual laboratories. Thus, the Proteomics/Oxidative Stress Core will play an important role in allowing investigators to analyze alterations in proteins with age and will be a valuable asset for studies involved in assessing the role of oxidative damage in biological samples. The Specific Aims of the Proteomics/Oxidative Stress Core are as follows: 1.-Identification and quantification of proteins in various sub-cellular compartments during aging. 2.-Measurement of oxidative damage to lipids and DNA through analysis of F2-isoprostanes/isofurans and 8-oxo-2-deoxyguanosine (oxoSdG), respectively. 3.-Developmental application of the novel global internal standard technology (GIST) method for mass spectrometric study of the proteome of cells/tissues.

蛋白质组学/氧化应激核心的目标有两个。首先，我们将为研究人员提供设备和技术援助，以鉴定新发现的来自各种组织和细胞隔间的蛋白质，并分析蛋白质水平的变化。分析和发现特定细胞间隔中与年龄相关的蛋白质，可能会为了解衰老过程中不可或缺的途径或代谢过程提供重要的新见解。作为第二项功能，我们将使用最新和最敏感的方法和技术手段来测量细胞和组织中脂质和DNA的氧化损伤。随着年龄的增长，细胞成分氧化损伤的积累被认为是一种 与年龄相关的生理和病理改变的主要因素。因此，准确测量氧化损伤的终点是至关重要的。上述测量需要昂贵的设备和技术专长，以防止这些类型的分析成为个别实验室的常规测量。因此，蛋白质组学/氧化应激 CORE将在允许研究人员分析蛋白质随年龄变化方面发挥重要作用，并将成为参与评估生物样本中氧化损伤作用的研究的宝贵资产。蛋白质组学/氧化应激核心的具体目标如下： 1.-衰老过程中不同亚细胞室中蛋白质的鉴定和定量。 2.-分别通过分析F2-异前列腺素/异呋喃和8-氧-2-脱氧鸟苷(OxoSdG)来测量对脂质和DNA的氧化损伤。 3.开发应用新的全球内部标准技术(GIST)方法，用于细胞/组织蛋白质组的质谱学研究。