CORE--PROTEOMICS/GENOMICS RESEARCH

骨骼肌萎缩是增龄性骨质疏松症、脊髓损伤、神经功能丧失和肌肉废用模型的重要病理过程。这个蛋白质组学和基因组学核心设施(PGCF)的目的是提供最先进的实验资源和开发新的程序，以帮助理解骨质疏松症发生的分子遗传和生化机制以及恢复的生物相互作用。这些方法为了解骨骼肌萎缩发生和恢复的分子和遗传机制提供了基本信息，包括 年龄相关性骨质疏松症(UTMB)和肌肉虚弱(JHU)、脊髓损伤和偏瘫中风(UM)以及心血管疾病(WF)，以及导致康复和康复的复杂分子生物学过程。所有这些研究领域都是许多Pepper OAIC的主要兴趣所在，并有助于统一这些中心的调查。PGCF有三个具体目标：AIM，1重点是发展和改进供Pepper OAIC调查人员使用的蛋白质组和基因组学高通量技术；Aim 2，重点是继续和扩大与Pepper OAIC调查人员正在进行的合作；Aim 3，重点是在分子基因组学和蛋白质组学领域培训年轻研究人员。PGCF的一个重要功能是提供快速、 对受肌肉萎缩影响的生物过程和对恢复至关重要的生物过程进行有效和最先进的调查和识别。可以识别与骨骼肌病理相关的全球基因表达、细胞器(线粒体)基因表达和生物发生、蛋白质池水平的变化以及蛋白质修饰等过程，从而为理解肌肉萎缩和恢复的分子机制提供所需的基本信息。这些研究最终为药物干预措施的设计提供了信息。

Skeletal muscle atrophy is an important pathological process in the sarcopenia of advancing age, spinal cord injury, and models of enervation and muscle disuse. The purpose of this Proteomics and Genomics Core Facility (PGCF) is to provide state-of-the art experimental resources and the development of new procedures that facilitate understanding the molecular genetic and biochemical mechanisms of sarcopenia development and the biological interactions of recovery. These approaches provide basic information on understanding the molecular and genetic mechanisms of development and recovery of skeletal muscle atrophies that include age-associated sarcopenia (UTMB) and muscle frailty (JHU), spinal cord injury and hemiparetic stroke (UM), and cardiovascular disease (WF), and the complex molecular biological processes leading to rehabilitation and recovery. All of these areas of research are of major interest of many of the Pepper OAICs and serve to unify the investigations at these centers. There are three Specific Aims of the PGCF: Aim, 1 focuses upon the development and improvement of proteomics and genomics high throughput technologies for use by Pepper OAIC investigators; Aim 2, focuses upon the continuation and extension of the on-going collaborations with Pepper OAIC investigators; Aim 3, focuses upon the training of young investigators in areas of molecular genomics and proteomics. An important function of the PGCF is to provide rapid, efficient and state-of-the art investigation and identification of the biological processes affected by the muscle atrophies and those essential to recovery. Such processes as global gene expression, organellar (mitochondrial) gene expression and biogenesis, changes in protein pool levels, and protein modifications associated with skeletal muscle pathology can be identified and thereby provide the basic information needed to understand the molecular mechanisms of muscle atrophy and recovery. These studies ultimately provide information for the design of pharmaceutical interventions.