CORE--PROTEOMICS/GENOMICS RESEARCH

核心--蛋白质组学/基因组学研究

• 批准号: 6847270
• 负责人: JOHN NMN PAPACONSTANTINOU
• 金额: $ 17.79万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6847270
• 关键词: aging; biomedical facility; gene expression; molecular pathology; musculoskeletal regeneration; proteomics; sarcopenia; spinal cord injury

Skeletal muscle atrophy is an important pathological process in the sarcopenia of advancing age, spinal cord injury, and models of enervation and muscle disuse. The purpose of this Proteomics and Genomics Core Facility (PGCF) is to provide state-of-the art experimental resources and the development of new procedures that facilitate understanding the molecular genetic and biochemical mechanisms of sarcopenia development and the biological interactions of recovery. These approaches provide basic information on understanding the molecular and genetic mechanisms of development and recovery of skeletal muscle atrophies that include age-associated sarcopenia (UTMB) and muscle frailty (JHU), spinal cord injury and hemiparetic stroke (UM), and cardiovascular disease (WF), and the complex molecular biological processes leading to rehabilitation and recovery. All of these areas of research are of major interest of many of the Pepper OAICs and serve to unify the investigations at these centers. There are three Specific Aims of the PGCF: Aim, 1 focuses upon the development and improvement of proteomics and genomics high throughput technologies for use by Pepper OAIC investigators; Aim 2, focuses upon the continuation and extension of the on-going collaborations with Pepper OAIC investigators; Aim 3, focuses upon the training of young investigators in areas of molecular genomics and proteomics. An important function of the PGCF is to provide rapid, efficient and state-of-the art investigation and identification of the biological processes affected by the muscle atrophies and those essential to recovery. Such processes as global gene expression, organellar (mitochondrial) gene expression and biogenesis, changes in protein pool levels, and protein modifications associated with skeletal muscle pathology can be identified and thereby provide the basic information needed to understand the molecular mechanisms of muscle atrophy and recovery. These studies ultimately provide information for the design of pharmaceutical interventions.

骨骼肌萎缩是增龄性肌肉减少症、脊髓损伤、失神经和肌肉废用模型的重要病理过程。该蛋白质组学和基因组学核心设施（PGCF）的目的是提供最先进的实验资源和新程序的开发，以促进了解肌肉减少症发展的分子遗传和生化机制以及恢复的生物学相互作用。这些方法提供了了解骨骼肌萎缩的发展和恢复的分子和遗传机制的基本信息，包括 年龄相关性肌肉减少症（UTMB）和肌肉虚弱（JHU）、脊髓损伤和轻偏瘫性中风（UM）和心血管疾病（WF），以及导致康复和恢复的复杂分子生物学过程。所有这些研究领域都是许多胡椒OAIC的主要兴趣，并有助于统一这些中心的调查。PGCF有三个具体目标：目标1，专注于开发和改进蛋白质组学和基因组学高通量技术，供Pepper OAIC研究人员使用;目标2，专注于继续和扩展与Pepper OAIC研究人员正在进行的合作;目标3，专注于分子基因组学和蛋白质组学领域的年轻研究人员的培训。PGCF的一个重要功能是提供快速， 对受肌肉萎缩影响的生物学过程和对恢复至关重要的生物学过程进行有效和最先进的研究和鉴定。这些过程如全局基因表达、细胞器（线粒体）基因表达和生物发生、蛋白质库水平的变化以及与骨骼肌病理学相关的蛋白质修饰，可以被识别，从而提供理解肌肉萎缩和恢复的分子机制所需的基本信息。这些研究最终为药物干预的设计提供了信息。