CRYSTALLOGRAPHIC STUDIES OF FRAGMENTS FROM REGULATED MYOSIN

描述（由申请人提供）：MacCHESS是大分子晶体学的同步辐射研究资源，其总体目标是通过创新技术研发和用户支持推进结构生物学的前沿。MacCHESS利用国家科学基金会对康奈尔高能同步加速器源（CHESS）的投资，该源维护同步辐射实验室，以及基本粒子物理实验室（LEPP），该实验室操作存储环。MacCHESS的技术发展是由涉及广泛的大分子的核心和合作研究项目推动的。除了进行技术研发和核心研究外，MacCHESS还为大分子晶体学提供专业仪器，并为用户提供培训和支持。MacCHESS已成为世界上最具生产力的大分子晶体学机构之一，在过去的五年中，由于MacCHESS相关的技术研发，核心和合作研究和服务，发表了近437篇论文。其中超过72篇论文发表在高知名度期刊《科学》、《自然》和《细胞》上。三个CHESS站，A-1，F-1和F-2，用于大分子晶体学实验。在接下来的五年里，MacCHESS的技术研发将集中在（1）微观晶体学，（2）膜蛋白晶体学，（3）大单位细胞结构，（4）高分辨率衍射，（5）高通量晶体学，（6）晶体学软件和定相技术以及（7）晶体学光束线硬件开发。这些技术研发工作中的每一项都是由合作研究人员提出的具有挑战性的问题所驱动的，这些研究人员研究了当前医学和生物学非常感兴趣的分子和复合物的原子结构，包括膜蛋白，调节蛋白复合物，核酸/蛋白复合物，蛋白质药物相互作用和病毒。MacCHESS研究所产生的技术发展免费提供给科学界，并通过会议、研讨会、出版物、网络分发和与其他同步加速器来源的合作传播。

DESCRIPTION (provided by applicant): MacCHESS, a synchrotron radiation Research Resource for macromolecular crystallography, has the overall goal of advancing the frontiers of structural biology through innovative technical research and development and user support. MacCHESS leverages the National Science Foundation investments in the Cornell High Energy Synchrotron Source (CHESS), which maintains the synchrotron radiation laboratory, and the Laboratory for Elementary Particle Physics (LEPP), which operates the storage ring. MacCHESS technical developments are driven by both Core and Collaborative research projects involving a broad range of macromolecules. In addition to performing technical R&D and Core research, MacCHESS provides specialized instrumentation for macromolecular crystallography and a staff for user training and support. MacCHESS has established itself as one of the most productive facilities in the world for macromolecular crystallography, with almost 437 papers published during the past five years as a result of MacCHESS related technical research and development, Core and Collaborative research and Service. More than 72 of these papers were published in the high visibility journals, Science, the Nature journals, and Cell. Three CHESS stations, A-1, F-1 and F-2, are used for macromolecular crystallography experiments. During the next five years, MacCHESS technical R&D will focus on (1) microcrystallography, (2) membrane protein crystallography, (3) large unit cell structures, (4) ultrahigh resolution diffraction, (5) high throughput crystallography, (6) crystallographic software and phasing techniques and (7) crystallographic beamline hardware development. Each of these technical R&D efforts is driven by the challenging problems presented by collaborating investigators who study the atomic structure of molecules and complexes of great current interest to medicine and biology, including membrane proteins, regulatory protein complexes, nucleic acid/protein complexes, protein drug interactions and viruses. Technical developments resulting from MacCHESS research are freely available to the scientific community and disseminated through meetings, workshops, publications, web distributions and collaborations with other synchrotron sources.