GOLGI ALPHA-MANNOSIDASE II AND CEX INHIBITOR COMPLEXES AT HIGH RESOLUTION

高分辨率高尔基体α-甘露糖苷酶 II 和 CEX 抑制剂复合物

• 批准号: 7181027
• 负责人: DAVID P ROSE
• 金额: $ 5.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181027
• 关键词: biomedical resource; structural biology

DESCRIPTION (provided by applicant): MacCHESS, a synchrotron radiation Research Resource for macromolecular crystallography, has the overall goal of advancing the frontiers of structural biology through innovative technical research and development and user support. MacCHESS leverages the National Science Foundation investments in the Cornell High Energy Synchrotron Source (CHESS), which maintains the synchrotron radiation laboratory, and the Laboratory for Elementary Particle Physics (LEPP), which operates the storage ring. MacCHESS technical developments are driven by both Core and Collaborative research projects involving a broad range of macromolecules. In addition to performing technical R&D and Core research, MacCHESS provides specialized instrumentation for macromolecular crystallography and a staff for user training and support. MacCHESS has established itself as one of the most productive facilities in the world for macromolecular crystallography, with almost 437 papers published during the past five years as a result of MacCHESS related technical research and development, Core and Collaborative research and Service. More than 72 of these papers were published in the high visibility journals, Science, the Nature journals, and Cell. Three CHESS stations, A-1, F-1 and F-2, are used for macromolecular crystallography experiments. During the next five years, MacCHESS technical R&D will focus on (1) microcrystallography, (2) membrane protein crystallography, (3) large unit cell structures, (4) ultrahigh resolution diffraction, (5) high throughput crystallography, (6) crystallographic software and phasing techniques and (7) crystallographic beamline hardware development. Each of these technical R&D efforts is driven by the challenging problems presented by collaborating investigators who study the atomic structure of molecules and complexes of great current interest to medicine and biology, including membrane proteins, regulatory protein complexes, nucleic acid/protein complexes, protein drug interactions and viruses. Technical developments resulting from MacCHESS research are freely available to the scientific community and disseminated through meetings, workshops, publications, web distributions and collaborations with other synchrotron sources.

描述（由申请人提供）：MacCHESS 是一种用于大分子晶体学的同步辐射研究资源，其总体目标是通过创新的技术研发和用户支持来推进结构生物学的前沿。 MacCHESS 利用国家科学基金会对康奈尔高能同步加速器源 (CHESS) 和基本粒子物理实验室 (LEPP) 的投资，前者负责维护同步辐射实验室，后者负责运营存储环。 MacCHESS 的技术发展由涉及广泛大分子的核心和协作研究项目推动。除了进行技术研发和核心研究外，MacCHESS 还提供用于大分子晶体学的专业仪器以及用于用户培训和支持的人员。 MacCHESS 已成为世界上生产力最高的大分子晶体学设施之一，在过去五年中，由于 MacCHESS 相关技术研发、核心和协作研究及服务，发表了近 437 篇论文。其中超过 72 篇论文发表在高知名度期刊《Science》、《Nature》和《Cell》上。三个 CHESS 站 A-1、F-1 和 F-2 用于大分子晶体学实验。未来五年，MacCHESS技术研发将重点关注（1）微晶体学、（2）膜蛋白晶体学、（3）大晶胞结构、（4）超高分辨率衍射、（5）高通量晶体学、（6）晶体学软件和定相技术以及（7）晶体学束线硬件开发。每项技术研发工作都是由合作研究人员提出的挑战性问题驱动的，这些研究人员研究当前医学和生物学领域非常感兴趣的分子和复合物的原子结构，包括膜蛋白、调节蛋白复合物、核酸/蛋白质复合物、蛋白质药物相互作用和病毒。 MacCHESS 研究成果可免费提供给科学界，并通过会议、研讨会、出版物、网络发行以及与其他同步加速器来源的合作进行传播。