Unraveling oceanic multi-element cycles using single cell ionomics
使用单细胞离子组学揭示海洋多元素循环
基本信息
- 批准号:2875388
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:英国
- 项目类别:Studentship
- 财政年份:2023
- 资助国家:英国
- 起止时间:2023 至 无数据
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Oceanic elemental cycles are strongly influenced by marine microbes, including phytoplankton. Although it has long been recognised that the elemental composition of microbes is a key determinant of these cycles, analytical limitations mean current understanding is typically based on observations of limited numbers of elements averaged across mixed communities. Appreciation of potentially important heterogeneities at individual and population levels and a more holistic understanding of multi-element cycling is thus lacking. Consequently, single-organism/cell analysis is a fast-growing field, but many techniques are hampered by low throughput. We aim to apply a new analytical tool (Time of Flight Inductively Coupled Plasma Mass Spectrometry, ToF-ICPMS) to facilitate rapid multi-element analysis of individual marine phytoplankton cells and potentially higher trophic levels. This project will focus on the key question of how variability in (co-)limiting nutrient availability influences the multi-elemental cellular composition of non-limiting nutrients, which has significant consequences for understanding coupled nutrient cycling. Although this problem has been addressed in specific cases on an element-by-element basis, we lack true multi-element ('ionomic') treatments, which also account for the heterogeneity of mixed natural communities. The project will thus facilitate a deeper understanding of multi-elemental ocean biogeochemical cycles.
海洋元素循环受到包括浮游植物在内的海洋微生物的强烈影响。虽然人们早就认识到微生物的元素组成是这些循环的关键决定因素,但分析的局限性意味着目前的理解通常是基于对混合群落中有限数量的平均元素的观察。因此,缺乏对个体和种群水平上潜在的重要异质性的认识以及对多元素循环的更全面的理解。因此,单生物/细胞分析是一个快速发展的领域,但许多技术受到低通量的阻碍。我们的目标是应用一种新的分析工具(飞行时间电感耦合等离子体质谱,ToF-ICPMS)来促进单个海洋浮游植物细胞和潜在的更高营养水平的快速多元素分析。该项目将重点关注(共同)限制性养分有效性的可变性如何影响非限制性养分的多元素细胞组成的关键问题,这对理解耦合营养循环具有重要意义。虽然这个问题已经在特定的情况下逐个元素的基础上得到了解决,但我们缺乏真正的多元素(“经济学”)处理,这也解释了混合自然群落的异质性。因此,该项目将有助于更深入地了解多元素海洋生物地球化学循环。
项目成果
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其他文献
吉治仁志 他: "トランスジェニックマウスによるTIMP-1の線維化促進機序"最新医学. 55. 1781-1787 (2000)
Hitoshi Yoshiji 等:“转基因小鼠中 TIMP-1 的促纤维化机制”现代医学 55. 1781-1787 (2000)。
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LiDAR Implementations for Autonomous Vehicle Applications
- DOI:
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2021 - 期刊:
- 影响因子:0
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吉治仁志 他: "イラスト医学&サイエンスシリーズ血管の分子医学"羊土社(渋谷正史編). 125 (2000)
Hitoshi Yoshiji 等人:“血管医学与科学系列分子医学图解”Yodosha(涉谷正志编辑)125(2000)。
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Effect of manidipine hydrochloride,a calcium antagonist,on isoproterenol-induced left ventricular hypertrophy: "Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,K.,Teragaki,M.,Iwao,H.and Yoshikawa,J." Jpn Circ J. 62(1). 47-52 (1998)
钙拮抗剂盐酸马尼地平对异丙肾上腺素引起的左心室肥厚的影响:“Yoshiyama,M.,Takeuchi,K.,Kim,S.,Hanatani,A.,Omura,T.,Toda,I.,Akioka,
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