Unraveling oceanic multi-element cycles using single cell ionomics

使用单细胞离子组学揭示海洋多元素循环

• 批准号: 2875388
• 金额: --
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2875388
• 关键词: Unraveling; oceanic; multi; element; cycles

Oceanic elemental cycles are strongly influenced by marine microbes, including phytoplankton. Although it has long been recognised that the elemental composition of microbes is a key determinant of these cycles, analytical limitations mean current understanding is typically based on observations of limited numbers of elements averaged across mixed communities. Appreciation of potentially important heterogeneities at individual and population levels and a more holistic understanding of multi-element cycling is thus lacking. Consequently, single-organism/cell analysis is a fast-growing field, but many techniques are hampered by low throughput. We aim to apply a new analytical tool (Time of Flight Inductively Coupled Plasma Mass Spectrometry, ToF-ICPMS) to facilitate rapid multi-element analysis of individual marine phytoplankton cells and potentially higher trophic levels. This project will focus on the key question of how variability in (co-)limiting nutrient availability influences the multi-elemental cellular composition of non-limiting nutrients, which has significant consequences for understanding coupled nutrient cycling. Although this problem has been addressed in specific cases on an element-by-element basis, we lack true multi-element ('ionomic') treatments, which also account for the heterogeneity of mixed natural communities. The project will thus facilitate a deeper understanding of multi-elemental ocean biogeochemical cycles.

海洋元素循环受到海洋微生物的强烈影响，包括浮游植物。尽管长期以来一直认识到微生物的元素组成是这些循环的关键决定因素，但分析的局限性意味着目前的理解通常是基于对混合群落中平均的有限数量元素的观察。因此，缺乏对个人和种群一级潜在的重要异质性的认识，也缺乏对多元素循环的更全面的了解。因此，单个生物/细胞分析是一个快速发展的领域，但许多技术因吞吐量低而受到阻碍。我们的目标是应用一种新的分析工具(飞行时间电感耦合等离子体质谱，ToF-ICPMS)来促进对单个海洋浮游植物细胞和潜在更高营养水平的多元素快速分析。本项目将集中于关键问题，即限制养分供应的可变性如何影响非限制养分的多元素细胞组成，这对理解耦合养分循环具有重要意义。虽然这个问题已经在特定情况下逐个元素地解决了，但我们缺乏真正的多元素(离子)处理，这也解释了混合自然群落的异质性。因此，该项目将有助于更深入地了解多种元素的海洋生物地球化学循环。