FUNCTIONAL STUDIES OF GLUTATHIONE TRANSFERASES

谷胱甘肽转移酶的功能研究

• 批准号: 7181925
• 负责人: JULIAN P. ADAMS
• 金额: $ 0.42万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181925
• 关键词: biomedical resource; structural biology

DESCRIPTION (provided by applicant): We will continue to operate BioCARS, a nationally-available research resource for synchrotron-based macromolecular crystallography at the Advanced Photon Source, Argonne National Laboratory. The facility contains three experimental stations, one served by either an undulator or a wiggler insertion device source and two served by a bending magnet source, all of which can be used simultaneously. All stations and the associated control areas are embedded in a BL3-level biosafety facility, to permit the safe study of, for example, pathogenic human viruses. Research by users has successfully and productively concentrated on de novo structure determination by MAD phasing; on the collection of accurate, high resolution, monochromatic data; on the study of crystals with large unit cell dimensions; and on time-resolved crystallography on time scales down to a few nanoseconds. We will continue to emphasize these areas and to support the most challenging user experiments, through our experienced technical and scientific staff. In a facility that is unique nationally and one of three internationally, we will further develop time-resolved crystallography in both core and collaborative research, largely on the insertion device station. Advances in this scientific technique have led to much broader user interest in applying it to a wide variety of systems, since it offers new insights into biological mechanism and function. Upgrades will be made to the X-ray beam lines, both to replace those older components damaged by radiation and by heating and to afford smaller focal spots that are better matched to the smaller crystals that users wish to examine. Detector and data acquisition and control software will be improved, both to support collaborative projects in structural genomics that demand high data throughput, and to enable telecrystallography, in which the user can participate effectively in all aspects of the synchrotron experiment but need not be physically present at BioCARS. Strategies for improving the diffraction quality of crystals at both room and cryogenic temperatures by annealing will be implemented. In all cases, these developments are aimed at enhancing the throughput of excellent, high-resolution diffraction data and solved scientific problems, by satisfied users.

描述（由申请人提供）：我们将继续运营 BioCARS，这是阿贡国家实验室先进光子源的全国性同步加速器大分子晶体学研究资源。该设施包含三个实验站，一个由波荡器或摆动器插入装置源提供服务，两个由弯曲磁体源提供服务，所有这些都可以同时使用。所有站点和相关控制区域均嵌入 BL3 级生物安全设施中，以便安全研究人类致病病毒等。 用户的研究已成功且富有成效地集中于通过 MAD 定相从头确定结构；收集准确、高分辨率、单色数据；研究具有大晶胞尺寸的晶体；以及时间尺度缩小到几纳秒的时间分辨晶体学。我们将继续强调这些领域，并通过我们经验丰富的技术和科学人员支持最具挑战性的用户实验。在这个全国独一无二、国际上三个设施之一的设施中，我们将在核心研究和协作研究方面进一步开发时间分辨晶体学，主要是在插入装置站上。这项科学技术的进步引起了更广泛的用户兴趣，将其应用于各种系统，因为它提供了对生物机制和功能的新见解。 X射线束线将进行升级，以替换那些因辐射和加热而损坏的旧组件，并提供更小的焦点，以更好地匹配用户希望检查的较小晶体。探测器以及数据采集和控制软件将得到改进，既支持需要高数据吞吐量的结构基因组学合作项目，又支持远程晶体学，其中用户可以有效地参与同步加速器实验的各个方面，但无需亲自出现在 BioCARS 中。将实施通过退火提高室温和低温下晶体衍射质量的策略。在所有情况下，这些发展都旨在提高优质、高分辨率衍射数据的吞吐量，并解决科学问题，让用户满意。