Core--Molecular Pathology

核心--分子病理学

• 批准号: 6901551
• 负责人: MARY L HIXON
• 金额: $ 14.3万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6901551
• 关键词: biomedical facility; environmental toxicology; immunocytochemistry; in situ hybridization; light microscopy; molecular pathology

The projects included in this Superfund application will require a Molecular Pathology Core to allow scientists to characterize various exposure paradigms to complex chemical mixtures, at the molecular level, and assess the environmental health consequences associated with such exposures. Hence, the ultimate goal of the Molecular Pathology Core is to provide a state-of-theart facility for the evaluation of pathological alterations associated with exposure to complex chemical mixtures. This goal will be achieved by the following Specific Aims: Specific aim 1. The appointing of a qualified director who will be responsible for the management of the Molecular Pathology Core and a histotechnician for the processing of samples; Specific aim 2. Through the acquisition of a high quality microscope, tissue arrayer, and autostainer to examine molecular alterations; and Specific aim 3. By the gradual institution of a fee-for-service structure which will ensure the long-term viability of the Molecular Pathology Core Facility. The core plans to acquire a multiheaded light microscope with digital projection capability which will be used as the major teaching and diagnostic microscope for the facility. The acquisition of a tissue arrayer and autostainer for immunohistochemistry and in situ hybridization analyses should allow investigators to determine the expression and localization of specific genes and/or proteins of interest to the proposed research projects. The institution of a Molecular Pathology Core will provide essential scientific resources for understanding the underlying disease processes associated with coexposures.

本超级基金申请中包括的项目将需要一个分子病理学核心，使科学家能够在分子水平上描述复杂化学混合物的各种暴露模式，并评估与此相关的环境健康后果。 暴露。因此，分子病理学核心的最终目标是提供一个国家的最先进的设施，用于评估与暴露于复杂的化学混合物相关的病理变化。这一目标将通过以下具体目标实现：具体目标1。任命一名合格的主任，负责管理分子病理学中心，并任命一名组织技术员处理样本;具体目标2。通过获得高质量的显微镜、组织排列仪和自动染色仪来检查分子改变;和具体目标3。通过逐步建立收费服务结构，确保分子病理学核心设施的长期生存能力。核心计划获得一个具有数字投影能力的多头光学显微镜，将用作该设施的主要教学和诊断显微镜。获得用于免疫组织化学和原位杂交分析的组织排列仪和自动染色仪应使研究人员能够确定拟议研究项目感兴趣的特定基因和/或蛋白质的表达和定位。分子病理学中心的建立将为理解与共同暴露相关的潜在疾病过程提供必要的科学资源。