Research Support Core D: Molecular Pathology Core

分子病理学核心提供生物医学和工程研究人员在 SBRP在布朗大学的技术专长和必要的科学设备，以评估和 根据暴露范例诊断从纳米到生物体水平的病理变化 范围从简单的化学暴露到更复杂的化学混合物。核心D使 研究者应用组织病理学、免疫组织化学和免疫细胞学方法， 通过各种显微技术观察形态。核心D的优势包括专业化 包括连续和/或分步切片的全胚胎包埋技术。核心D提供薄 电子和透射显微镜的切片能力，用于观察 纳米材料（工程）和细胞/组织（生物医学）。常规程序包括处理和 石蜡包埋标本，塑料和冷冻切片，用于光学显微镜检查。常规染色 这些方法如定期希夫染色和苏木精和曙红染色。的方法论 特殊染色程序，如凋亡细胞免疫染色（TUNEL），损伤诱导灶 （yH 2AX）、非程序性DNA合成（溴脱氧尿苷/Ki 67）和免疫细胞染色（凝集素和免疫球蛋白）。 GR 1）的毒性。核心D也有一个新收购的振动切片机 软组织是组织冷冻和/或石蜡包埋的替代物。Core D的另一个独特之处是 它为仪器提供教育和实践培训。ScanScope CS系统来自 Aperio Technologies是一种虚拟显微镜，使各种SBRP实验室能够收集详细的 扫描用于组织样本的图像分析。先进的算法软件使研究人员能够 处理图像，以识别和量化形态结构，从而减少 个人分级组织病理学切片。总之，核心D将继续提供这些资源 通过以下目标：1。提供组织学评估方面的专业知识和咨询 样品和援助超级基金项目成员和布朗大学社区的成员， 分子病理学技术的应用。2.为研究人员、学生和技术人员提供培训 工作人员在各种技术和组织形态的解释。3.为了预测新兴市场的需求， 病理学方法，并建立其使用的专业知识。4.促进SBRP之间的合作 调查人员和整个研究界。

The Molecular Pathology Core provides both biomedical and engineering researchers within the SBRP at Brown University the technical expertise and scientific equipment necessary to evaluate and diagnose pathological alterations from the nano to the organismal level following exposure paradigms ranging from the simple chemical exposure to the more complex chemical mixture. Core D enables investigators to apply histopathological, immunohistochemical and immunocytological methods in order to visualize morphology through various microscopic techniques. The strengths of Core D include specialized techniques for whole embryo embedding including serial and/or step sectioning. Core D provides thin sectioning capability for electron and transmission microscopy for the visualization of interactions between nanomaterials (engineering) and cells/tissue (biomedical). Routine procedures include the processing and embedding of specimens for paraffin, plastic and frozen sectioning for light microscopy. Routine staining procedures such as Periodic Schiff's Staining and Hematoxylin and Eosin stains. The methodology for specialty staining procedures such as immunostaining for apoptotic cells (TUNEL), damaged induced foci (yH2AX), unscheduled DNA synthesis (bromodeoxyuridine/Ki67), and staining for immune cells (lectin and GR1) following toxicant exposure are available. Core D also has a newly acquired vibratome for sectioning soft tissue an alternative for freezing and/or paraffin embedding of tissue. Another unique aspect of Core D is that it provides educational and hands on training for its instrumentation. The ScanScope CS system from Aperio Technologies is a virtual microscope which enables various SBRP laboratories to collect detailed scanning for image analysis of tissue specimens. The advanced algorithm software allows researchers to process images in order to identify and quantify morphological structures, thus reducing variability between individuals grading histopathology sections. In summary, Core D will continue to provide these resources through the following aims: 1. To provide expertise and consultation in the assessment of histological samples and assistance to Superfund project members and members of the Brown University community in the use of techniques in molecular pathology. 2. To provide training for investigators, students and technical staff in various technologies and interpretation of tissue morphology. 3. To anticipate the need for emerging pathology methodologies and establish expertise in their use. 4. To facilitate collaboration between SBRP investigators and the research community at large.