Research Support Core D: Molecular Pathology Core

分子病理学核心为生物医学和工程研究人员提供 布朗大学SBRP所需的技术专长和科学设备，以评估和 根据暴露范例诊断从纳米到生物水平的病理变化 从简单的化学暴露到更复杂的化学混合物。酷睿D支持 调查人员将应用组织病理学、免疫组织化学和免疫细胞学方法 通过各种显微技术使形态可视化。Core D的优势包括专门的 全胚胎植入技术，包括连续和/或阶梯式切片。核心D提供精简 用于可视化相互作用的电子显微镜和透射显微镜的切片能力 纳米材料(工程学)和细胞/组织(生物医学)。例行程序包括处理和 石蜡切片、塑料切片和光学显微镜冰冻切片标本的包埋。常规染色 定期希夫氏染色和苏木精伊红染色等程序。的方法论 特殊染色程序，如凋亡细胞(TUNEL)免疫染色、受损诱导灶 (YH2AX)，程序外DNA合成(溴脱氧尿苷/Ki67)，免疫细胞染色(凝集素和 Gr1)可供选择。D芯也有一个新获得的用于分段的振动器 软组织是冷冻和/或石蜡包埋组织的一种替代方法。核心D的另一个独特之处是 它为它的乐器提供教育和实践培训。ScanScope CS系统来自 Aperio Technologies是一种虚拟显微镜，使各种SBRP实验室能够收集详细的 扫描组织标本的图像分析。先进的算法软件使研究人员能够 处理图像，以便识别和量化形态结构，从而减少 个人对组织病理学切片进行分级。总而言之，核心D将继续提供这些资源 通过以下目标：1.在组织学评估方面提供专业知识和咨询 向超级基金项目成员和布朗大学社区成员提供的样本和援助 分子病理学中技术的使用。2.为调查人员、学生和技术人员提供培训 从事各种技术和组织形态解释的工作人员。3.预见新兴市场的需求 病理学方法，并在其使用方面建立专门知识。4.促进SBRP之间的协作 调查人员和整个研究社区。

The Molecular Pathology Core provides both biomedical and engineering researchers within the SBRP at Brown University the technical expertise and scientific equipment necessary to evaluate and diagnose pathological alterations from the nano to the organismal level following exposure paradigms ranging from the simple chemical exposure to the more complex chemical mixture. Core D enables investigators to apply histopathological, immunohistochemical and immunocytological methods in order to visualize morphology through various microscopic techniques. The strengths of Core D include specialized techniques for whole embryo embedding including serial and/or step sectioning. Core D provides thin sectioning capability for electron and transmission microscopy for the visualization of interactions between nanomaterials (engineering) and cells/tissue (biomedical). Routine procedures include the processing and embedding of specimens for paraffin, plastic and frozen sectioning for light microscopy. Routine staining procedures such as Periodic Schiff's Staining and Hematoxylin and Eosin stains. The methodology for specialty staining procedures such as immunostaining for apoptotic cells (TUNEL), damaged induced foci (yH2AX), unscheduled DNA synthesis (bromodeoxyuridine/Ki67), and staining for immune cells (lectin and GR1) following toxicant exposure are available. Core D also has a newly acquired vibratome for sectioning soft tissue an alternative for freezing and/or paraffin embedding of tissue. Another unique aspect of Core D is that it provides educational and hands on training for its instrumentation. The ScanScope CS system from Aperio Technologies is a virtual microscope which enables various SBRP laboratories to collect detailed scanning for image analysis of tissue specimens. The advanced algorithm software allows researchers to process images in order to identify and quantify morphological structures, thus reducing variability between individuals grading histopathology sections. In summary, Core D will continue to provide these resources through the following aims: 1. To provide expertise and consultation in the assessment of histological samples and assistance to Superfund project members and members of the Brown University community in the use of techniques in molecular pathology. 2. To provide training for investigators, students and technical staff in various technologies and interpretation of tissue morphology. 3. To anticipate the need for emerging pathology methodologies and establish expertise in their use. 4. To facilitate collaboration between SBRP investigators and the research community at large.