SCIENTIFIC CORE

药物滥用的分子靶点及其相关细胞内信号通路的研究 已经产生了大量关于与这些药物相关的细胞扰动的信息。的 联合研究将在现有知识的基础上扩展严格的细胞生物学， 药物治疗后神经元细胞的分子、生物化学、行为和电生理学研究。这些 研究有可能提供更深入的了解药物依赖的原因，以及阐明 药理学干预的可能新靶点。 科学核心将是一个致力于促进项目中提出的实验的中心 需要使用转基因动物、酵母双杂交筛选和各种关键生物试剂。 在这些项目中概述的实验需要创造和维持大量的基因， 改良动物必要的转基因小鼠的创建和表征，以及维持 这些和所有其他转基因小鼠是特定目标I的核心。帮助识别和描述 与滥用药物靶向的信号分子相关的新型蛋白质-蛋白质相互作用，核心将设计 并在Specific Aim II中进行酵母双杂交筛选。此外，科学核心将是一个关键的来源， 实验所需的各种试剂。关键蛋白质、酶和抗体 将在特异性目标III中产生，而新的多克隆抗体，包括磷酸化状态特异性抗体， 抗体，将在特定目标IV中产生。

Investigations into the molecular targets of drugs of abuse and their associated intracellular signaling pathways have yielded a wealth of information regarding the cellular perturbations associated with these drugs. The combined studies will extend upon this existing knowledge with rigorous cell biological, molecular, biochemical, behavioral, and electrophysiological studies of neuronal cells upon drug treatment. These studies have the potential to provide greater insights into the causes of drug dependence as well as to elucidate possible novel targets for pharmacological intervention. The Scientific Core will be a center devoted to facilitating those experiments proposed in the Projects that require the use of genetically modified animals, yeast two-hybrid screens, and various key biological reagents. The experiments outlined in these Projects require the creation and maintenance of large numbers of genetically modified animals. The creation and characterization of necessary transgenic mice, and the maintenance of these and all other genetically modified mice is Specific Aim I of the Core. To help identify and characterize novel protein-protein interactions relevant to signaling molecules targeted by drug of abuse, the Core will design and execute yeast two-hybrid screens in Specific Aim II. In addition, the Scientific Core will be a key source of various reagents needed for the experiments. Key proteins, enzymes, and antibodies will be produced in Specific Aim III, while new polyclonal antibodies, including phosphorylation statespecific antibodies, will be produced in Specific Aim IV.