Core C: Biobank Core

核心 C：生物样本库核心

• 批准号: 7037893
• 负责人: SARAH P THAYER
• 金额: $ 10.77万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7037893
• 关键词: adenocarcinoma; biomedical facility; histopathology; immunocytochemistry; pancreatic duct; tissue resource /registry

A major obstacle to research in pancreatic cancer has been lack of access to high-quality annotated specimens of human tissues from all stages of PDAC. This difficulty is due to the relative inaccessibility of the pancreas and its prominent fibrous component, which make it technically difficult to obtain samples of sufficient heoplastic cellularity for many analytic approaches. The overall goal of the BioBank Core will be established to assist PPG member laboratories in the accrual and centralization of biologic resources that are critically required for pancreatic cancer research. Specific aims are directed at: 1) maintaining a centralized, molecularly annotated human-derived murine xenograft core which'can enrich and amplify the neoplastic cell population while maintaining the genotypic and histologic character closest to that of the parent human cancer; 2) providing a molecularly annotated cell-line repository. To accomplish these aims we will take advantage of our immediate access to human surgical specimens and mouse tumors to provide P01 investigators with biological materials that have been subjected to extensive characterization at the histologic and genetic levels (in collaboration with the Experimental Pathology Core). These materials include: serially passaged xenografts created from human pancreatic cancer specimens from primary and metastatic cancers; PDAC cell lines from our mouse models with varying tumor suppressor gene mutations; and primary mouse pancreatic ductal cells with various mutational profiles. Together, these resources should provide standardized cellular systems to facilitate experimental studies. The Core also maintains a repository of nearly all available PDAC cell lines; these have been subjected to high-resolution CGH and expression profiling, enabling investigators to retrieve needed cell lines with an appropriate genomic profile. The Core will interact closely with the Projects of the P01 to provide the biological materials and aid in the design and interpretation of experiments employing the xenografts and primary cells. The Core will seek to ensure a high level of quality control of specimens and standardization of protocols to ensure the effective translation of results between P01 Projects.

胰腺癌研究的一个主要障碍是缺乏高质量的注释 PDAC 各个阶段的人体组织标本。这种困难是由于相对难以接近 胰腺及其突出的纤维成分，这使得在技术上很难获得胰腺样本 对于许多分析方法来说有足够的造血细胞结构。生物样本库核心的总体目标是 成立的目的是协助 PPG 成员实验室积累和集中生物资源， 胰腺癌研究至关重要。具体目标是：1）保持 集中的、分子注释的人源鼠异种移植核心，可以丰富和放大 肿瘤细胞群同时保持最接近肿瘤细胞的基因型和组织学特征 母体人类癌症； 2）提供分子注释的细胞系存储库。为了实现这些目标 我们将利用我们立即获得人类手术标本和小鼠肿瘤的优势来提供 P01 研究人员使用已在实验室进行了广泛表征的生物材料 组织学和遗传水平（与实验病理学核心合作）。这些材料 包括：从原发性和胰腺癌的人类胰腺癌标本中连续传代的异种移植物 转移性癌症；来自我们小鼠模型的 PDAC 细胞系，具有不同的肿瘤抑制基因突变； 以及具有各种突变特征的原代小鼠胰腺导管细胞。这些资源共同应 提供标准化的细胞系统以促进实验研究。核心还维护一个 几乎所有可用的 PDAC 细胞系的存储库；这些都经过了高分辨率 CGH 处理 表达谱，使研究人员能够检索具有适当基因组谱的所需细胞系。 核心将与 P01 项目密切互动，提供生物材料和援助 使用异种移植物和原代细胞的实验的设计和解释。核心将寻求 确保标本的高水平质量控制和方案的标准化，以确保有效 P01 项目之间结果的翻译。