权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

BIOBANK CORE

生物样本库核心

基本信息

批准号：
8052123
负责人：
SARAH P THAYER
金额：
$ 12.7万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-01-01 至 2011-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8052123
关键词：
Animal Model Archives Biocompatible Materials Bioinformatics Biological Biological Models Biology Cancer cell line Candidate Disease Gene Cell Line Cells Collaborations Computer Simulation Disease Ensure Experimental Pathology Extinction (Psychology)Generations Genetic Genomics Histologic Human Image Laboratories Lethal Genes MEKs Malignant neoplasm of pancreas Metastatic Lesion Methods Modeling Molecular Molecular Profiling Operative Surgical Procedures Pancreatic Ductal Adenocarcinoma Paraffin Embedding Parents Pathway interactions Patients Pharmaceutical Preparations Primary Lesion Protocols documentation Quality Control Research Personnel Resistance Resolution Resources Screening procedure Signal Transduction Specimen Standardization System Testing Tissue Microarray Tissue Sample Tissues Translations Tumor Cell Line Work Xenograft Model Xenograft procedure advanced disease anticancer research biobank cell growth design experience human tissue imaging probe member novel novel therapeutic intervention pancreatic cancer cells programs repository research study resistance mechanism response small hairpin RNA transcriptomics tumor tumor xenograft

项目摘要

The BioBank Core will assist project member laboratories in the accrual and centralization of biologic resources that are critically required for pancreatic cancer research. We will take advantage of our immediate access to human surgical specimens to provide P01 investigators with biological materials that have been subjected to extensive characterization at the histologic (in collaboration with the Experimental Pathology Core) and genetic levels. The Core will collect, initiate, maintain, archive and record all human PDAC biologic resources. These materials include serially passaged xenografts created from primary and metastatic human pancreatic cancer specimens and a repository of PDAC cell lines. The xenograft tumors and cell lines have been subjected to high-resolution aCGH and expression profiling, enabling investigators to retrieve needed biologic materials with an appropriate genomic profile. Together, these resources should facilitate molecular studies and provide standardized cellular systems for performing experimental studies. Members of the BioBank Core have extensive experience in implementation of xenograft systems for testing novel therapeutic approaches and for molecular and biological studies. The Core will interact closely with the projects of the P01 to provide the biological materials and aid in the design and interpretation of experiments employing the xenografts and primary cells. The Core will seek to ensure a high level of quality control of specimens and standardization of protocols to ensure the effective translation of results between P01 projects.

BioBank Core将协助项目成员实验室积累和集中胰腺癌研究所需的生物资源。我们将利用我们直接获得人类手术标本的优势，为P01研究人员提供在组织学（与实验病理学核心合作）和遗传水平上进行广泛表征的生物材料。核心将收集、启动、维护、存档和记录所有人类PDAC生物资源。这些材料包括从原发性和转移性人胰腺癌标本和PDAC细胞系库中产生的连续传代异种移植物。异种移植肿瘤和细胞系已接受高分辨率aCGH和表达谱分析，使研究人员能够检索具有适当基因组谱的所需生物材料。总之，这些资源应该促进分子研究，并提供标准化的细胞系统进行实验研究。BioBank Core的成员在实施异种移植系统进行测试方面拥有丰富的经验新的治疗方法和分子和生物学研究。核心将与P01的项目密切合作，提供生物材料，并帮助设计和解释使用异种移植物和原代细胞的实验。核心将努力确保标本的高水平质量控制和方案的标准化，以确保P01项目之间的结果有效转化。