Multidisciplinary Program in Female /Male Reproductiion

描述（由申请人提供）：马里兰大学医学院生殖研究专业合作中心项目（SCCPRR）的目标是开展女性和男性生殖生物学的创新基础和临床研究，并应用从这些研究中获得的知识来提高对女性和男性生殖功能障碍和不育的病因和治疗的理解。本研究的主题是雌激素在调节灵长类动物胎儿卵巢和睾丸在子宫内发育过程中的成熟以及在月经周期中子宫内膜血管重塑中的作用。在项目1 （Gerald J. Pepe）中，将在狒狒中评估子宫内雌激素在规划胎儿卵巢卵泡和卵母细胞发育中的调节作用及其对成年期生育能力的影响。在项目II （Eugene D. Albrecht）中，将评估子宫内雌激素对狒狒胎儿睾丸和外管/附睾发育的调节作用及其对成年期生育能力的影响。Project III （Robert D. Koos）提出了一项临床与基础相结合的研究，旨在确定雌激素和黄体酮通过血管刺激因子和血管抑制因子的表达，在狒狒体内和体外人子宫内膜与血管内皮细胞和壁细胞共培养中对子宫内膜血管生成和血管重构的作用。我们将与马里兰大学SCCPRR、东弗吉尼亚医学院、匹兹堡大学SCCPRR和约翰霍普金斯大学SCCPRR的人类和非人类灵长类动物生殖生物学专家合作。每个研究项目都将大量利用免疫细胞化学核心（Gloria E. Hoffman）进行调节因子和受体的细胞定位/表达。本次SCCPRR的科学工作将通过关注雌激素在生殖系统发育成熟中的作用，使用狒狒作为非人灵长类动物模型，以及建立合作研究的传统来实现。在狒狒灵长类动物模型中获得的发现有望转化为人类，以推进人类生殖生物学的知识。

DESCRIPTION (provided by applicant): The objective of the Specialized Cooperative Centers Program in Reproduction Research (SCCPRR) at the University of Maryland School of Medicine is to conduct innovative basic and clinical research on female and male reproductive biology and to apply the knowledge gained from these studies to improve understanding of the etiology and treatment of reproductive dysfunction and infertility in women and men. The thematic focus of this proposal is the role that estrogen plays in regulating maturation of primate fetal ovaries and testes during intrauterine development and vascular remodeling of the uterine endometrium during the menstrual cycle. In Project I (Gerald J. Pepe), the regulatory role of estrogen in utero in programming fetal ovarian follicular and oocyte development and the impact this has on fertility in adulthood will be assessed in the baboon. In Project II (Eugene D. Albrecht), the regulatory role of estrogen in utero in programming baboon fetal testicular and excurrent ductule/epididymal development and the impact this has on fertility in adulthood will be assessed. A combined clinical-basic research study is proposed in Project III (Robert D. Koos), to determine the role of estrogen and progesterone, acting via expression of angiostimulatory and angioinhibitory factors, on endometrial angiogenesis and vascular remodeling in vivo in baboons and in vitro in cocultures of human endometrial and vascular endothelial and mural cells. We will collaborate with experts in human and nonhuman primate reproductive biology at the University of Maryland SCCPRR, Eastern Virginia Medical School, the University of Pittsburgh SCCPRR, and the Johns Hopkins University SCCPRR. Each of the research projects will draw heavily upon the immunocytochemistry Core (Gloria E. Hoffman) for the cellular localization/expression of regulatory factors and receptors. Integration of the scientific effort in this SCCPRR will be achieved through the thematic focus on estrogen's role in developmental maturation of the reproductive system, use of the baboon as a nonhuman primate model, and an established tradition of collaborative research. The discoveries obtained in the baboon primate model are expected to translate to the human to advance knowledge of human reproductive biology.