Impact of pregnancy-specific glycoproteins on development and ageing

我们的目的是研究遗传变异如何影响PSG基因调控，从而影响滋养层细胞和衰老细胞的细胞表型。我们将利用尖端的干细胞和测序技术与表观基因组学和功能测定，汇集滋养层基因调控（布兰科实验室），人口基因组学/表观基因组学（贝尔实验室）和衰老（阿尔托斯实验室）的专业知识。更具体地说，我们的目标是：1。深入研究了一组诱导多能干细胞（iPSC）系上PSG基因座的遗传学。检测PSG基因变异对iPSC源性滋养层细胞的影响.测试PSG遗传变异对iPSC衍生的衰老细胞模型的影响

We aim to investigate how genetic variation impacts PSG gene regulation and consequently on cellular phenotypes in trophoblast and senescent cells. We will leverage cutting-edge stem cell and sequencing technologies with epigenomics and functional assays, bringing together expertise in trophoblast gene regulation (Branco lab), population genomics/epigenomics (Bell lab) and ageing (Altos Labs). More specifically, we aim to:1. Deeply characterise the genetics of the PSG locus on a set of induced pluripotent stem cell (iPSC) lines.2. Test the impact of PSG genetic variation on iPSC-derived trophoblast cells.3. Test the impact of PSG genetic variation on iPSC-derived cellular models of senescence