Waste Stream Valorisation: Solving sustainable chemical production

废物流增值：解决可持续化学品生产问题

• 批准号: 2881252
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2881252
• 关键词: Waste; Stream; Valorisation; Solving; sustainable

Industrial chemical production is reliant on fossil fuel feed stocks and processes, which are unsustainable with a devastating effect on the climate. Bio-based production represents a promising alternative, where sustainable carbon sources and engineered microorganisms can be used to produce a wide variety of commodity chemicals. This project aims to develop a pipeline to drastically decrease the time and costs to create bacterial strains capable of producing the chemicals we need.One of the biggest hurdles to implementing biobased solutions is the cost of feedstocks used for bacterial growth and production of chemicals. This project will exemplify the use of alternative carbon sources, generate consistent feedstocks from waste streams and develop new ways of using high-potential, under-utilised carbon streams to accelerate the switch to global sustainability.Objectives1. Identifying the chassis:Investigating common E.coli production strains for their ability to use advantaged carbon sources. In parallel a comparable analysis of strains of E. coli already adapted to alternative carbon sources will be carried out.2. Identifying waste streamsAn initial techno-economic analysis will be carried out on a selection of Agricultural/highly heterogeneous waste streams. After which viable waste streams will be fed into the platform for chassis selection and tested.3. Chassis engineeringAfter identification of a strain(s) that perform well on advantaged or alternative carbon sources, in house genomic sequencing will be used to elucidate the genomic context of this "fitness". Combined with Twig's computational biology and data science expertise, a genome engineering approach will be taken to increase carbon utilization.4. Exemplification with Twig pathwayAny identified chassis or modified strain that shows potential will be used to express Twigs proprietary constructs and subjected to fermentation analysis in a small scale continuous system.

工业化学品生产依赖于化石燃料原料和工艺，这是不可持续的，对气候造成破坏性影响。生物基生产是一种有前途的替代方案，可持续的碳源和工程微生物可用于生产各种商品化学品。该项目旨在开发一个管道，以大幅减少时间和成本，创造能够生产我们所需的化学品的细菌菌株。实施生物基解决方案的最大障碍之一是用于细菌生长和化学品生产的原料成本。该项目将鼓励使用替代碳源，从废物流中产生稳定的原料，并开发利用高潜力、利用率低的碳流的新方法，以加速向全球可持续发展的转变。目标1。确定底盘：调查常见的大肠杆菌生产菌株使用碳源的能力。同时，对大肠杆菌菌株进行了比较分析。已经适应替代碳源的大肠杆菌将进行.将对选定的农业/高度异质性废物流进行初步技术经济分析。之后，可行的废物流将被送入平台进行底盘选择和测试。底盘工程在鉴定出对碳源或替代碳源表现良好的菌株后，将使用内部基因组测序来阐明这种“适应性”的基因组背景。结合Twig的计算生物学和数据科学专业知识，将采取基因组工程方法来提高碳利用率。Twig途径的例证任何鉴定的底盘或显示潜力的修饰菌株将用于表达Twigs专有构建体，并在小规模连续系统中进行发酵分析。