Bioproducing succinic acid from hydrolysate of biomass waste in a biofilm reactor

在生物膜反应器中从生物质废物的水解产物中生物生产琥珀酸

• 批准号: 2881637
• 金额: --
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2881637
• 关键词: Bioproducing; succinic; acid; hydrolysate; biomass

Succinic acid is an important platform chemical and has been identified as one of the top potential chemical building blocks for the future. Succinic acid is currently manufactured mainly by petrochemical processes. A move from fossil fuel to renewable raw materials for sustainable development has been started for succinic acid fermentation towards a biobased economy. However, the bioproduction of succinic acid is mainly restricted to conventional feedstock with a batch operation mode at the moment, resulting in low productivity and feedstock competition for food. To explore the economic and environmental feasibility of succinic acid bioproduction with widely available biomass waste, the key technical challenges such as inhibition from biomass hydrolysate and low productivity have to be overcome. Given the aggregation characteristics of some organic acid-producing species such as Actinobacillus succinnogenes for succinic acid production under some conditions, this project aims to explore the aggregation potential of Actinobacillus succinnogenes and develop either attached biofilm or self-aggregated microbial granules for continuous bioproduction of succinic acid from hydrolysate of biomass waste. The inhibition from hydrolysate and succinic acid in fermentation broth could be alleviated due to the three-dimensional structure formed by cell attachment to support or self-aggregation. Furthermore, this biofilm-based technology can not only retain higher biomass in reactors but also enable continuous fermentation with improved productivity. Apart from biofilm-based process development for continuous fermentation of succinic acid, the analysis of energy performance and CO2 fixation in the succinic acid production process will be carried out in this project for the comparison with conventional suspended cells based batch fermentation with glucose as well as the evaluation of the environmental sustainability of the newly developed process.This project would suit candidates with a strong degree in Biotechnology, Bioengineering, Biochemical Engineering, Biology, Chemical Engineering, Environmental Engineering, or closely related disciplines. If you wish to discuss any details of the project

琥珀酸是一种重要的平台化学物质，已被确定为未来最有潜力的化学基石之一。琥珀酸目前主要由石油化工工艺生产。从化石燃料转向可持续发展的可再生原料，琥珀酸发酵朝着生物基经济的方向发展。然而，目前琥珀酸的生物生产主要局限于常规原料，采用间歇式操作模式，导致生产效率低，原料竞争激烈。为了探索利用广泛利用的生物质废弃物生产琥珀酸的经济和环境可行性，必须克服诸如生物质水解液抑制和低生产率等关键技术挑战。鉴于琥珀酸放线菌等有机产酸菌在一定条件下的聚集特性，本项目旨在探索琥珀酸放线菌的聚集潜力，开发附着生物膜或自聚集微生物颗粒，用于生物质废弃物水解液连续生产琥珀酸。发酵液中水解液和琥珀酸的抑制作用可因细胞附着或自聚集形成的三维结构而减轻。此外，这种基于生物膜的技术不仅可以在反应器中保留更高的生物量，而且可以提高生产效率，实现连续发酵。本项目除了开发基于生物膜的琥珀酸连续发酵工艺外，还将对琥珀酸生产工艺的能量性能和CO2固定进行分析，与传统的基于悬浮细胞的葡萄糖间歇发酵工艺进行比较，并对新工艺的环境可持续性进行评价。本项目适合具有生物技术、生物工程、生化工程、生物学、化学工程、环境工程或相关学科学位的候选人。如果你想讨论项目的任何细节