Sustainable Succinic Acid Production using an Integrated Electrochemical Bioreactor and Renewable Feedstock

项目概要：LUCRA将利用欧盟未充分利用和丰富的城市固体废物和木材废物作为原料，使用循环生物经济生物精炼方法，以高产量大规模生产平台构建块生物基化学品，具有重要的工业最终用户利益。它将提供优化和可扩展的“突破性的第一种”创新技术，以在TRL7中通过新型电化学提取琥珀酸（SA）实现从中试到大规模集成发酵的阶段性变化，这将促进其工业实施，商业模式和市场可行性。这将通过循环利用上述可再生资源作为原料来实现，这将在很大程度上减少对化石资源的依赖，同时实现可持续的SA生产路线。为了满足工业水平的需求，LUCRA将优化不同原料的水解工艺，以获得高效的纤维素和半纤维素转化。酶混合物将被证明旨在最大限度地水解可发酵的碳水化合物含量与低抑制剂的生产。将富含糖的水解液作为发酵原料，在电化学膜生物反应器中用于高效提取和生产SA。SA的原位提取将通过阴离子交换膜实现，导致同时酸化、浓缩、用于pH调节的较低NaOH消耗和用于SA纯化的较少单元操作。为了证明生产的SA能够直接与石化工艺中的化石对应物竞争，将以半工业规模生产不同的聚氨酯分散体和树脂。LUCRA实施的工艺旨在降低生物SA的成本，并将证明与传统SA相比，温室气体减少50%。

Project summary: Project summary LUCRA will exploit the EU’s underutilized and abundant, Municipal Solid Waste and Wood waste as feedstocks for the large scale production of platform building block biobased chemicals at high yield with significant industrial end-user interest using a circular bioeconomy biorefinery approach. It will provide optimized and scaleable ‘breakthrough first of a kind’ innovative technology to create a step change from pilot to large scale integrated fermentation with novel electrochemical extraction of succinic acid (SA) at TRL7 that will facilitate its industrial implementation, business models and market feasibility. This will be achieved by the circular utilization of the mentioned renewable resources as feedstocks which will reduce to a large extent the dependence on fossil resources and achieves at the same time a sustainable SA production route. To fit the needs of the industrial level, LUCRA will optimize the hydrolysis process of the different feedstocks to obtain efficient cellulose and hemicellulose conversion. Enzymatic cocktails will be demonstrated aiming to maximize hydrolysis of fermentable carbohydrate content with low inhibitors production. The crude sugar-rich hydrolysates will be used as fermentation feedstock in an electrochemical membrane bioreactor for efficient extraction and production of SA. In situ extraction of SA will be achieved through an anion exchange membrane resulting in simultaneous acidification, concentration, lower NaOH consumption for pH regulation and less unit operations for SA purification. To demonstrated the produced SA that will be able to compete directly their fossil-based counterparts from petrochemical processes, different polyurethane dispersions and resins will be produced at semi-industrial scale. The process implemented in LUCRA targeting to reduce the cost of bio-SA and will demonstrate an 50% GHG reduction against conventional SA