Somatic Coliphage and Viral Risk Considering Treatment Competence in Low Income Settings

考虑低收入环境中的治疗能力的体细胞噬菌体和病毒风险

• 批准号: 2881954
• 金额: --
• 依托单位: CRANFIELD UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2881954
• 关键词: Somatic; Coliphage; Viral; Risk; Considering

Differences between virus interaction with other treatment processes are well documented. For example, free chlorine has a wide variety of efficacy against different strains of Coxsackievirus (Kahler et la. 2010). Viruses have a predominantly negative surface charge that varies depending on the water matrix. Recent investigations have shown the effect of strain level differences to the virus genome and capsid on the surface charge and hydrophobicity which may determine their removal during the coagulation/flocculation stage. There is a dearth of knowledge regarding how these differences manifest and influence interactions between the viruses and coagulants. I aim to shed lights on these aspect by testing different strains of coliphages (somatic and F-specific) representing different pathogenic viruses prevalent in different water matrices to address the following questions:1. What is the mechanism of interaction between different viruses, coagulants/flocculants, and mechanisms of coagulation/flocculation?2. How effective is the optimum coagulant/flocculant dose in removing or inactivating viruses by disrupting the viral genome and capsid?3. Do all viruses respond differently to a specific coagulant dose at specific conditions?4. What is the response of viruses to different coagulant doses and how it varies with change in other parameters such as pH and temperature?5. As most of the viruses present in water are negatively charged. What is the any relation between virus removal/inactivation and zeta potential?

病毒与其他治疗过程相互作用的差异已得到充分证明。例如，游离氯对不同的柯萨奇病毒株具有多种功效（Kahler et la. 2010年）。病毒主要具有负表面电荷，其取决于水基质而变化。最近的研究表明，病毒基因组和衣壳的菌株水平差异对表面电荷和疏水性的影响，这可能决定了它们在凝聚/絮凝阶段的去除。关于这些差异如何表现和影响病毒和凝血剂之间的相互作用，缺乏知识。我的目标是通过测试代表不同水基质中流行的不同致病性病毒的不同大肠杆菌菌株（体细胞和F特异性）来阐明这些方面，以解决以下问题：1。不同病毒、凝聚剂/絮凝剂之间的相互作用机制以及凝聚/絮凝机制是什么？2.通过破坏病毒基因组和衣壳来去除或灭活病毒的最佳凝血剂/絮凝剂剂量的有效性如何？3.在特定条件下，所有病毒对特定剂量的凝血剂的反应是否不同？4.病毒对不同剂量的凝血剂的反应如何，以及它如何随其他参数（如pH值和温度）的变化而变化？5.因为大多数存在于水中的病毒都是带负电荷的。病毒去除/灭活与zeta电位之间有什么关系？