Function of Conserved Structures in Related Coliphage RNA's
相关大肠杆菌噬菌体 RNA 中保守结构的功能
基本信息
- 批准号:9316501
- 负责人:
- 金额:$ 23万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:1994
- 资助国家:美国
- 起止时间:1994-06-01 至 1998-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Jacobson 9316501 Dr. Jacobson and coworkers study the shape of large genomic RNAs in the RNA coliphage system. These molecules have a dual function: they carry the genetic information coding for viral genes; and in addition, hydrogen bonding between distant regions of the molecules regulate viral growth, controlling both the expression of viral proteins and viral RNA replication. The detailed conformation of the molecules will be studied in order to understand the structural mechanisms used in regulation. Electron microscopy is used as a primary tool. This method allows one to see large structural features in the RNA and to measure changes in the shape and relative stability of these structures in mutant RNAs that have been created by genetic engineering. The structural changes that are observed can be correlated with changes in the growth patterns of mutant viruses. The current work focuses on the conformation of genomic RNA from coliphage QB. Dr. Jacobson's studies demonstrated for the first time that the molecules contain two types of structural domains. One type is unusually stable; the other is highly variable and can switch between alternative conformations. Both types of structures appear to be utilized in the regulation of viral protein synthesis. Two of the stable domains appear to suppress translational initiation of viral proteins. In addition, stable domains may serve as structural anchors (much like door hinges) that limit the formation of alternative structures in the variable regions of the molecule. In addition, computer modeling will be used to understand RNA conformation. A new method was developed to detect likely stable structures as well as likely variable regions of an RNA molecule. The results obtained by modeling are consistent with electron microscopic studies with both native and mutant RNAs. The new approach is of general interest and will be used by investigators in many disciplines. %%% Many unanswered questions remain about the rol e of RNA structure in gene regulation in RNA coliphages. These can now be addressed with modern methods of analysis. Answers to these questions will provide fundamental insights into the properties of all large mRNA molecules, and are therefore relevant in understanding mechanisms governing growth, evolution, and the regulation of gene expression in disease causing plant and animal viruses. ***
Jacobson 9316501 Jacobson博士及其同事研究了RNA大肠杆菌噬菌体系统中大基因组RNA的形状。这些分子具有双重功能:它们携带编码病毒基因的遗传信息;此外,分子远端区域之间的氢键调节病毒生长,控制病毒蛋白质的表达和病毒RNA的复制。将研究分子的详细构象,以了解调控中使用的结构机制。 电子显微镜被用作主要工具。这种方法允许人们看到RNA中的大结构特征,并测量通过基因工程产生的突变RNA中这些结构的形状和相对稳定性的变化。观察到的结构变化可以与突变病毒生长模式的变化相关。 目前的工作集中在大肠杆菌噬菌体QB基因组RNA的构象。雅各布森博士的研究首次证明了分子包含两种类型的结构域。 一种是异常稳定的;另一种是高度可变的,可以在不同的构象之间切换。 这两种类型的结构似乎都用于调节病毒蛋白质合成。两个稳定结构域似乎抑制病毒蛋白的翻译起始。此外,稳定结构域可以作为结构锚(很像门铰链),限制在分子的可变区中形成替代结构。此外,计算机建模将用于理解RNA构象。开发了一种新方法来检测RNA分子可能的稳定结构以及可能的可变区。通过建模获得的结果与天然和突变RNA的电子显微镜研究一致。这种新方法具有普遍意义,将被许多学科的研究人员所使用。 关于RNA结构在RNA大肠杆菌基因调控中的作用,仍有许多未解之谜。 现在可以用现代分析方法来解决这些问题。 这些问题的答案将提供对所有大mRNA分子的性质的基本见解,因此与理解植物和动物病毒中的生长、进化和基因表达调控机制有关。 ***
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Ann Jacobson其他文献
Further data on a weighted average model for judgment in a lifted weight task
- DOI:
10.3758/bf03209513 - 发表时间:
1968-03-01 - 期刊:
- 影响因子:1.700
- 作者:
Norman H. Anderson;Ann Jacobson - 通讯作者:
Ann Jacobson
Interdisciplinary Care at CKD Centers Assists With BP Control, Anemia Management, and Diabetes Control and Slows the Progression of CKD
- DOI:
10.1053/j.ackd.2009.01.003 - 发表时间:
2009-03-01 - 期刊:
- 影响因子:
- 作者:
Michele Cici;Jeffery Lautman;Terri Pierson;Ann Jacobson;Veronica Sumodi;Karen Fagnilli;Kelly Dion - 通讯作者:
Kelly Dion
3 Relationship of Health Literacy, Knowledge, and Patient Activation with Self-Management in Persons with Heart Failure
- DOI:
10.1016/j.hrtlng.2017.04.023 - 发表时间:
2017-05-01 - 期刊:
- 影响因子:
- 作者:
Ann Jacobson;Veronica Sumodi;Donna Walker;Lori DeJohn;Kelly Dion;Hua-Li Lin Tai;Linda Blankenship;Donna Ross - 通讯作者:
Donna Ross
Ann Jacobson的其他文献
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{{ truncateString('Ann Jacobson', 18)}}的其他基金
Comparative Analysis of Structure in RNAs of Related RNA Coliphages
相关RNA大肠杆菌噬菌体RNA结构的比较分析
- 批准号:
9019123 - 财政年份:1991
- 资助金额:
$ 23万 - 项目类别:
Continuing Grant
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