MULTIPHOTON CONFOCAL MICROSCOPE UPGRADES: CELL & DEVELOPMENTAL BIOLOGY

多光子共焦显微镜升级：细胞

• 批准号: 7335037
• 负责人: JAMES E BEAR
• 金额: $ 14.52万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2007-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335037
• 关键词: electromagnetic radiation; lasers

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This is a proposal to upgrade our one- and multi-photon laser scanning microscope system that is based on our Zeiss 510 NLO system. The upgrade consists of 1) the Chameleon, an easily use femtosecond pulse Ti:Sapphire laser to replace our aging and difficult to use Ar pumped Mira laser; 2) Zeiss Physiology software for the 510 system that allows more sophisticated image processing and analysis; 3) workstation software to duplicate the 510 analysis functionality on standalone computers so that our heavily used 510 system can be used for collecting data. Our Zeiss 510 system is in a heavily used multi-user facility that provides image acquisition and analysis for a wide variety of one- and multi-photon excitation projects. These upgrades are sought 1) to improve the reliability and ease of use of multi-photon excitation so that an average user can work independently; 2) greatly increase the speed of changing wavelengths of multi-photon excitation to allow novel experimental approaches; 3) provide increased software functionality on the 510 system; and 4) to make analysis functions available "off-line" so that the 510 system can be dedicated to data acquisition. Our facility includes two other laser scanning confocal microscopes, a Zeiss 410 and a BioRad 600, and a spinning disk confocal microscope, from Atto Instruments. Our microscope systems are used for a variety of projects by investigators from several laboratories.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：这是一项升级我们基于 Zeiss 510 NLO 系统的单光子和多光子激光扫描显微镜系统的提案。升级包括 1) Chameleon，一种易于使用的飞秒脉冲 Ti:Sapphire 激光器，用于取代我们老化且难以使用的 Ar 泵浦 Mira 激光器； 2) 适用于 510 系统的 Zeiss Physiology 软件，可实现更复杂的图像处理和分析； 3) 工作站软件在独立计算机上复制 510 分析功能，以便我们频繁使用的 510 系统可用于收集数据。我们的 Zeiss 510 系统位于一个频繁使用的多用户设施中，为各种单光子和多光子激发项目提供图像采集和分析。这些升级的目的是：1）提高多光子激发的可靠性和易用性，以便普通用户可以独立工作； 2）大大提高多光子激发波长的变化速度，以实现新颖的实验方法； 3）在510系统上提供增加的软件功能； 4) 使分析功能“离线”可用，以便 510 系统可以专用于数据采集。我们的设施包括另外两台激光扫描共焦显微镜（一台 Zeiss 410 和一台 BioRad 600），以及一台来自 Atto Instruments 的转盘共焦显微镜。我们的显微镜系统被多个实验室的研究人员用于各种项目。