NCRR: UCSC Acquisition of a Thermo Electron LTQ-Mass Spectrometer

描述（由申请人提供）：13名加州大学圣克鲁兹分校的教职员工共同开发了购买最先进的芬尼根LTQ质谱计系统（LTQ MS）的申请。该系统由一个高性能二维线性四极离子阱质谱仪（LTQ）组成。我们要求NCRR资助购买该系统，以极大地扩展和现代化我们的质谱MS设备，该设备致力于UCSC化学和生物化学系（CBC），分子，细胞和发育生物学（MCD）和环境毒理学（ETOX）的研究。共享的质谱设备目前有两台质谱仪，而另外两台质谱仪则安置在各个实验室，并可根据要求提供给生物医学研究学院。虽然这些工具都得到了很好的维护，但它们各自都有局限性，削弱了它们支持本可以在这所大学进行的研究的质量和广度的能力。如果没有额外的、更新的设备，我们为教师提供一流研究基础设施的能力将继续受到损害。这一问题在未来只会加剧，因为这四个部门的生物医学研究人员预计将在未来十年扩大50%。收购LTQ MS将在缓解这些问题方面发挥重要作用。十大主要用户的研究活动可分为两大领域。每一个都对基础生物科学以及相关的生物技术应用和疾病治疗具有深远的影响。广泛的主题领域包括：1)基于esi -线性离子阱的蛋白质组学研究复杂的生化过程；2)esi -线性离子阱方法理解信号转导途径。区域1的五个主要用户（Fink, Holman, Lokey， Millhauser和Smith）在项目中使用蛋白质组学方法，这取决于拟议仪器的分辨率和灵敏度。另外五个与Area 2相关的主要用户（Hartzog, Kellogg, Noller， Ottemann和Tamkun）利用该技术进行生化细胞机制表征。三名次要用户将使用该仪器研究蛋白质结构和功能，并阐明涉及毒理学的细胞机制。

DESCRIPTION (provided by applicant): Thirteen UCSC faculty members have come together to develop this application for the purchase of a state-of-the-art Finnigan LTQ Mass Spectrometer System (LTQ MS). This system consists of a high- performance 2D linear quadrupole ion trap mass spectrometer (LTQ). We are asking the NCRR to fund the purchase of this system to greatly expand and modernize our Mass Spectrometry MS Facility, which is dedicated to research in UCSC's Departments of Chemistry and Biochemistry (CBC), Molecular, Cell, and Developmental Biology (MCD), and Environmental Toxicology (ETOX). The shared MS Facility currently houses two mass spectrometers, while 2 additional mass spectrometers are housed in individual labs and available on request to the biomedical research faculty. While these instruments are well maintained, each has limitations that undercut its ability to support the quality and breadth of research that could otherwise be undertaken at this university. Without additional, updated equipment, our ability to provide our faculty with a first class research infrastructure will remain compromised. This problem will only become exacerbated in the future, as the biomedical research faculty of these four departments is projected to expand by 50% over the next decade. Acquisition of the LTQ MS will play a large role in alleviating these problems. The research initiatives of the ten major users can be divided into two broad areas. Each has far reaching implications for basic biological science, as well as for related biotechnology applications and the treatment of disease. The broad subject areas include: 1) ESI-Linear Ion-Trap based proteomics to study complex biochemical processes 2) ESI-Linear Ion-Trap approaches to understanding signal transduction pathways. The five major users in Area 1 (Fink, Holman, Lokey, Millhauser, and Smith) use proteomic methods on projects that depend on the resolution and sensitivity of the proposed instrument. Five other major users associated with Area 2 (Hartzog, Kellogg, Noller, Ottemann, and Tamkun) utilize this technology for biochemical cellular machinery characterization. Three Minor Users who will employ the instrument to investigate protein structure and function, and to clarify cellular mechanisms involved toxicology.