EPR SPECTROMETER: BIOENERGETICS OF HEART FAILURE

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：一组NIH资助的研究人员计划购买Bruker E680电子顺磁共振（EPR）光谱仪，以加强他们对各种生物医学问题的研究。该仪器将提供两个在整个上中西部的商业光谱仪中不可用的功能：（1）高场超导磁体和相关的高频（94 GHz，“W波段”）操作，以及（2）脉冲激励和相关的时域检测。有了这台仪器，商业EPR仪器经历了一场类似于80年代和90年代NMR的革命，随着更高频率和时域技术的出现，灵敏度和分辨率得到了显着提高，为分子结构，动力学和相互作用提供了更深入，更直接的物理见解。这产生了大量的研究机会，开辟了定位自旋标记（SDSL）和顺磁金属络合物分析的全新研究领域。这些仪器仅由布鲁克在德国制造，已经安装在整个欧洲的实验室中，但在美国安装的很少，在明尼苏达州、威斯康星州或爱荷华州还没有这样的仪器。因此，如果该区域的研究人员要继续在EPR应用方面保持高水平的生产力，就需要所要求的仪器，例如：肌球蛋白结构动力学（托马斯，Rayment，Titus和Nesmelov），心脏钙转运的调节（托马斯和Veglia），肌肉老化的生物物理学（汤普森和托马斯），金属蛋白质结构和机制（Lipscomb和Que），SNARE介导的膜融合（Shin），病毒DNA包装的结构动力学（安德森）和心力衰竭的生物能量学（Bache和Nesmelov）

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A group of NIH-funded investigators plans to acquire a Bruker E680 electron paramagnetic resonance (EPR) spectrometer, to enhance their research on a wide range of biomedical problems. This instrument will offer two features that are not available in a commercial spectrometer in the entire upper Midwest: (1) a high-field superconducting magnet and the associated high-frequency (94 GHz, "W-band") operation, and (2) pulsed excitation and the associated time-domain detection. With this instrument, commercial EPR instrumentation has undergone a revolution similar to that of NMR in the 80s and 90s, with the advent of higher frequencies and time-domain technology providing dramatic improvements in sensitivity and resolution, providing deeper and more direct physical insight into molecular structure, dynamics, and interactions. This has produced an explosion of research opportunities, opening up whole new areas of investigation in site-directed spin- labeling (SDSL) and analysis of paramagnetic metal complexes. These instruments, manufactured only by Bruker in Germany, have already been installed in labs throughout Europe, but very few have been installed in the United States, and there is no such instrument in Minnesota, Wisconsin, or Iowa. Thus the requested instrument is needed if researchers in this region are to continue their high level of productivity in EPR applications, such as the following: Myosin Structural Dynamics (Thomas, Rayment, Titus, and Nesmelov), Regulation of Cardiac Calcium Transport (Thomas and Veglia), Biophysics of Muscle Aging (Thompson and Thomas), Metalloprotein Structure and Mechanism (Lipscomb and Que), SNARE-mediated Membrane Fusion (Shin), Structural Dynamics of Viral DNA Packaging (Anderson), and Bioenergetics of Heart Failure (Bache and Nesmelov)