Comparative and functional genomics to unveil liver fluke sexual development.

比较和功能基因组学揭示肝吸虫的性发育。

• 批准号: 2884096
• 金额: --
• 依托单位: Aberystwyth University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2884096
• 关键词: Comparative; functional; genomics; unveil; liver

In this project I aim to utilise a mixture of computational biology including using large scale omics data and in vitro culture and manipulation of juvenile Fasciola hepatica to help uncover the sexual development of immature fluke. I will also be utilising single cell omics to reveal molecular factors present in individual cells of the parasite that could help to uncover the key genes involved in the sexual development of F. hepatica. The project will use a diverse range of tools, from gene expression manipulation, localisation of proteins and transcripts in the parasite tissues to high-throughput analyses of different phenotypic effects in genetically manipulated parasites. The techniques I will use and develop during the project will hopefully aim to answer a wide range of questions surrounding the sexual development of juvenile Fasciola and in turn help aid our understanding of the helminth's genomics. Utilising novel technology and cross disciplinary research may also lead to exciting prospects for uncovering potential novel targets for the control of fascioliasis.

在这个项目中，我的目标是利用计算生物学的混合物，包括使用大规模的组学数据和体外培养和操纵少年肝片吸虫，以帮助揭示未成熟的吸虫的性发育。我还将利用单细胞组学来揭示寄生虫个体细胞中存在的分子因子，这可能有助于揭示参与F。肝。该项目将使用多种工具，从基因表达操纵，寄生虫组织中蛋白质和转录本的定位到遗传操纵寄生虫中不同表型效应的高通量分析。我将在项目期间使用和开发的技术将有望回答围绕少年片形吸虫性发育的广泛问题，并反过来帮助我们了解蠕虫的基因组学。利用新技术和跨学科研究也可能导致发现控制片形吸虫病的潜在新靶点的令人兴奋的前景。