Combinatorial Use of Anti-HIV RNA-based Therapeutics

基于 RNA 的抗 HIV 疗法的组合使用

• 批准号: 7284685
• 负责人: John Joseph Rossi
• 金额: $ 41.97万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7284685
• 关键词: AIDS-Related Lymphoma; Acquired Immunodeficiency Syndrome; Address; Adjuvant; Animals; Antiviral Agents; Autologous; Biological Assay; Bone Marrow Transplantation; CD34 gene; Cells; Clinical Research; Clinical Trials; Condition; Controlled Study; Data; Development; Escape Mutant; Evaluation; Funding; Gene Expression; Gene Targeting; Gene-Modified; Genes; Goals; HIV; HIV Infections; Hematopoietic; Hematopoietic stem cells; Highly Active Antiretroviral Therapy; Human; In Vitro; Investigation; Lead; Lentivirus Vector; Life Cycle Stages; Localized; Macaca; Macaca nemestrina; MicroRNAs; Modeling; Modification; Monitor; Monkeys; Mutation; Patients; Pharmaceutical Preparations; Phase I Clinical Trials; Population; Pre-Clinical Model; Primates; Probability; Process; Proteins; RNA; RNA Interference; Research; Resistance; Safety; Site; Small RNA; Stem cells; System; T-Lymphocyte; Testing; Therapeutic; Time; Toxic effect; Transduction Gene; Transfection; Transgenes; Transplantation; Treatment Protocols; Universities; Vertebral column; Viral; Virus; Washington; base; cellular targeting; cellular transduction; combinatorial; concept; cost; gene therapy; in vivo; inhibitor/antagonist; interest; internal control; mutant; novel; prevent; programs; research study; simian human immunodeficiency virus; therapeutic gene; vector; viral RNA

DESCRIPTION (provided by applicant): The use of multi-drug regimens which target different HIV encoded proteins has dramatically changed the course of AIDS. The concept of highly active retroviral therapy (HAART) is to increase antiviral potency while minimizing the probability of mutant viral resistance. Despite the impact on AIDS, there are complications of HAART, including emerging resistant mutants, toxicities of HAART with other medications, and the long term cost of a lifetime of medication. An alternative or possible adjuvant to HAART therapy is the use of combinatorial gene therapy modification of hematopoietic cells. Research from this program has led to the development of a lentiviral vector harboring three different therapeutic genes targeting different steps in the HIV life cycle. This triple RNA inhibitor combination has proven to be the most potent of our tested anti-HIV approaches to date. This vector will enter a phase I clinical trial for hematopoietic stem cell transduction (HSC) of AIDS/lymphoma patient cells sometime in late 2006, and T-lymphocyte trials in 2007. The present proposal capitalizes upon the findings of the previous funding period, and proposes to develop new anti-HIV combinatorial RNAs with enhanced potency and safety to prevent or minimize the emergence of viral escape mutants. The proposed studies will explore a novel polycistronic micro RNA system as a possible platform for co-expressing combinations of anti-HIV siRNAs and nucleolar localizing inhibitory RNAs. The proposed studies incorporate novel anti-HIV targeting approaches which will be tested alone and in combinatorial fashion with already established inhibitory RNAs. Delivery of the inhibitory genes will be via lentiviral vector transduction of the combinatorial constructs into HSCs for in vitro and in vivo evaluations of anti-HIV efficacy and potential toxicities. The proposed studies will allow us to critically test the hypothesis that combinatorial gene therapy for treatment of HIV infection can be effected via genetically modified HSC transplantation. The in vivo testing will be conducted using transduced HSCs and bone marrow transplantation in the pigtail macaque (M. nemestrina) as part of a collaborative effort with Drs Kiem and Hu through the University of Washington Primate Center. The safety and efficacy of the constructs will be critically evaluated in this system. The Specific Aims of this study are: 1) Development of new strategies for multiplexing anti-HIV gene expression;2) Expression strategies and mechanism of action studies; 3) Evaluation of in vivo hematopoietic repopulation capabilities and resistance to viral challenge of vector transduced macaque CD34+ hematopoietic progenitor cells. These studies will provide the first detailed, in vivo investigations of antiviral RNAi and other inhibitory RNA combinations in a primate HSC gene therapy setting, and should provide the type of safety and efficacy data required to bring the lead combinatorial constructs to human clinical trials.

描述（由申请人提供）：靶向不同HIV编码蛋白的多药治疗方案的使用极大地改变了艾滋病的病程。高效逆转录病毒疗法（HAART）的概念是增加抗病毒效力，同时最大限度地减少突变病毒耐药性的可能性。尽管对艾滋病有影响，但HAART也有并发症，包括出现耐药突变体，HAART与其他药物的毒性，以及终生药物治疗的长期成本。HAART疗法的替代或可能的辅助是使用造血细胞的组合基因疗法修饰。该项目的研究导致了一种慢病毒载体的开发，该载体携带三种不同的治疗基因，靶向HIV生命周期的不同步骤。这种三重RNA抑制剂组合已被证明是迄今为止我们测试的抗HIV方法中最有效的。这种载体将在2006年晚些时候进入艾滋病/淋巴瘤患者细胞造血干细胞转导（HSC）的I期临床试验，并在2007年进入T淋巴细胞试验。目前的建议充分利用上一个资助期的研究结果，并建议开发新的抗艾滋病毒组合RNA，其效力和安全性更高，以防止或尽量减少病毒逃逸突变体的出现。该研究将探索一种新的多顺反子微RNA系统，作为共表达抗HIV siRNA和核仁定位抑制RNA组合的可能平台。拟议的研究纳入了新的抗HIV靶向方法，这些方法将单独测试，并与已经建立的抑制性RNA组合测试。抑制性基因的递送将通过组合构建体的慢病毒载体转导到HSC中，用于抗HIV功效和潜在毒性的体外和体内评价。拟议的研究将使我们能够批判性地检验治疗艾滋病毒感染的组合基因疗法可以通过基因修饰的HSC移植来实现的假设。将使用转导的HSC和猪尾猕猴（M. nemestrina）作为与Kiem博士和Hu博士通过华盛顿大学灵长类动物中心合作努力的一部分。将在该系统中对结构的安全性和有效性进行严格评价。本研究的具体目的是：1）开发用于多重抗HIV基因表达的新策略; 2）表达策略和作用机制研究; 3）评价载体转导的猕猴CD 34+造血祖细胞的体内造血重建能力和对病毒攻击的抗性。这些研究将提供灵长类HSC基因治疗环境中抗病毒RNAi和其他抑制性RNA组合的第一个详细的体内研究，并应提供将领先的组合构建体带入人类临床试验所需的安全性和有效性数据类型。