Development of a calcium-phosphate nanocomplex fluoride-based mouthrinse

描述（由申请人提供）：龋齿的形成和发展是一种疾病，影响了近80%的美国17岁人口。虽然自引入市售氟化物以来，在减少龋齿发病率方面取得了巨大成功，但改进现有技术的机会不仅存在，而且需求量很大。因此，鉴定可以与氟化物协同作用以增强再矿化并进一步防止牙列脱矿的新材料可以提供巨大的公共卫生效益。含氟漱口水是一种已被证明有效的氟化物输送方法，尤其适用于高危龋齿人群，包括戴牙套的人、口干症患者和需要修复的人。因此，本研究的长期目标是构建一种单一的多矿物质漱口水配方，该配方在磷酸钙纳米复合物和氟化物之间表现出稳定性，从而提供比单独使用氟化物更高水平的龋齿保护。我们假设这些磷酸钙纳米复合物可以通过机械化学球磨技术形成，因此与氟化物稳定。这种众所周知的技术不仅可以制造更小的颗粒尺寸，还可以改变研磨材料的化学特征，从而使其与通常的研磨方法明显不同。在NIDCR的第一阶段研究应用中，我们建议解决以下具体目标：1)使用易于扩展的新型机械化学球磨工艺在固态下完全合成许多表面活性剂-磷酸钙纳米复合物；2)测试纳米配合物加入碱性氟化钠漱口剂配方时的动力学和热力学稳定性；3)通过pH循环再矿化/脱矿模型和牙釉质氟摄取研究，评估最有前途的稳定纳米复合物氟漱口水配方的再矿化效果。

DESCRIPTION (provided by applicant): Caries formation and progression is a disorder that affects nearly 80% of the U.S. population by age 17. While great success has been achieved in reducing caries incidence since the introduction of commercially available fluoride, opportunities for improving the current art are not just available, they are in great demand. Therefore, identification of novel materials that could work synergistically with fluoride to enhance remineralization and further prevent demineralization of the dentition could provide a tremendous public health benefit. Fluoride mouthrinses are a proven and favorable method of fluoride delivery, especially for high-risk caries groups including those wearing braces, those with xerostomia, and those with restorations. Therefore, the long-term goal of this research is to construct a single multi-mineral mouthrinse formulation that manifests stability between calcium-phosphate nanocomplexes and fluoride to provide increased levels of protection against caries than would be observed with fluoride alone. We posit these calcium-phosphate nanocomplexes can be formed through a mechanochemical ball milling technique and, as a result, are stable with fluoride. This well-known technique not only creates smaller particle sizes but also modifies the chemical signatures of the milling material, thus setting it distinctly apart from usual milling methods. In this Phase I research application to the NIDCR, we propose to address the following Specific Aims: 1) Synthesize numerous surfactant-calcium phosphate nanocomplexes entirely in the solid-state using a novel mechanochemical ball milling process that is readily scaleable for commercial applications; 2) Test the kinetic and thermodynamic stability of the nanocomplex when added to basic sodium fluoride mouthrinse formulations; and 3) Evaluate the remineralization efficacy of the most promising stable nanocomplex-fluoride mouthrinse formulations using pH cycling remineralization/demineralization models and enamel fluoride uptake studies.